Chapter 5 Sequence Assembly: Assembling the Human Genome
Figure 5.1: The 23 sets of chromosomes in the human genome Courtesy of National Cancer Institute
Figure 5.2: (A) A normal deoxycytosine nucleotide used in DNA synthesis, and (B) a dideoxycytosine that would cause chain termination
Figure 5.3: A dideoxy sequencing reaction
Figure 5.4: Primer walking or directed sequencing of a genomic insert in a BAC vector
Figure 5.5: Shotgun sequencing
Figure 5.6: A DNA trace and computer-called base sequence: typical output from an automated sequencing run
Figure 5.7: Sample output from the CAP assembler
Figure 5.8: Assembly of overlapping DNA sequence fragments into a contig, showing how coverage can allow error correction
Figure 5.9: Schematic illustration of the sequence assembly and validation process
Figure 5.10: Graph of coverage values for each base position in a DNA sequence, with consecutive, high values indicating the possible misassembly of a repeated region
Figure 5.11: Graph representing overlaps between fragments as paths between nodes
Figure 5.12: Steps in finding a path to a sequence alignment