Exacerbated oxidative stress in the fasting liver according to fuel partitioning Thierry Wasselin 1,2, Sandrine Zahn 1,3, Yvon Le Maho 1,3, Alain Van Dorsselaer.

Slides:

Advertisements

Similar presentations

1 Chapter 43 Inborn Metabolic Defects of Lysosomes, Peroxisomes, Carbohydrates, Fatty Acids and Mitochondria Copyright © 2012, American Society for Neurochemistry.

Advertisements

Chapter 14 (Part 1) Electron transport. Chemiosmotic Theory Electron Transport: Electrons carried by reduced coenzymes are passed through a chain of.

Fermentations NADH must be oxidized to NAD + in order to oxidize glyceraldehyde-3-P In the absence of an electron transport chain pyruvate or a derivative.

KREBS CYCLE. Introduction Let us review fates of Pyruvate Depending on the oxidation state of the cell: Aerobic – converted to acetyl-CoA via TCA cycle.

Database Searches. Peptide mass fingerprinting digestMS Search HIT SCORE Protein X 1000 Protein Y 50 Protein Z 5 Protein X theoretical digestProtein Y.

Prentice Hall c2002Chapter 121 Chapter 12 - The Citric Acid Cycle The citric acid cycle is involved in the aerobic catabolism of carbohydrates, lipids.

Chapter 12 (part 1) Citric Acid Cycle. Gylcolysis TCA Cycle Electron Transport and Oxidative phosphorylation.

Chapter 12 (part 1) Citric Acid Cycle.

Figure 24-17Schematic representation of the thylakoid membrane showing the components of its electron-transport chain. Page 886.

Chapter 13 - The Citric Acid Cycle The citric acid cycle (tricarboxylic acid cycle) is amphibolic (both catabolic and anabolic) The cycle is involved in.

Do the Pathway Shuffle! M G1G1 G2G2 S Start here Induce! Revert! Horizontal Transfer Induce! Pick Copyright 2014 by Michael V. Osier. All rights reserved.

Beta oxidation of fatty acids takes place in the mitochondrial matrix for the most part. However, fatty acids have to be activated for degradation by coenzyme.

Dr.S.Chakravarty M.D.. Carbohydrates Fats 36+-/ Proteins recycling.

Energy Transfer.

Oxidative Decarboxylation of pyruvate and TCA cycle

Respiratory Substrates

Citric Acid Cycle What is it? Series of rxns that oxidize acetyl CoA to 2CO 2 in a manner that conserves the liberated free energy for ATP production Breakdown.

Respiration rate (nmol O 2 /min/mg protein) Succinate (mM) Supplementary Figure S1. Respiration rates of the mitochondria isolated from the florets of.

Final Review C483 Spring Replication.

Carbohydrate Metabolism Turning Sugar into Energy.

1 Lecture 6B – outline Mitochondrial function (e.g. hepatocytes) 1) citric acid cycle as an energy source a) pyruvate or  -ketoglutarate dehydrogenase.

~~ ~~ Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display GLUCOSE Pentose phosphate pathway Starts.

Biochemistry department

Ch. 20 Tricarboxylic acid cyle Student Learning Outcomes:

Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp Instructor: Kirill Popov.

CITRIC ACID CYCLE- discovered by Sir Hans Krebs in He was awarded Nobel Prize in Medicine Sir Hans KrebsSir Hans Krebs 1. The citric acid cycle (also.

Chapter 13 - The Citric Acid Cycle The citric acid cycle is involved in the aerobic catabolism of carbohydrates, lipids and amino acids Intermediates of.

9.2 The Process of Cellular Respiration

SURVEY OF BIOCHEMISTRY Citric Acid Cycle

Figure 1S. BSR homology. Exhaustive pairwise alignment using neighbour-joining phylogeny analysis by Clone Manager7 software shows the high homology of.

Laxman Yetukuri T : Modeling of Proteomics Data

Glycolysis 1. From glucose to pyruvate; step reactions; 3

Slide 1 Mosby items and derived items © 2012 by Mosby, Inc., an affiliate of Elsevier Inc. Chapter 16 Nutrition and Metabolism.

Pyruvate Carboxylase Reversing the final steps.

Substrate and oxidative phosphorylation. Substrate-level phosphorylation is a type of chemical reaction that results in the formation and creation of.

LEHNINGER PRINCIPLES OF BIOCHEMISTRY Sixth Edition David L. Nelson and Michael M. Cox © 2013 W. H. Freeman and Company CHAPTER 17 Fatty Acid Catabolism.

BC21D: Bioenergetics & Metabolism The formation of Acetyl Coenzyme A; Krebs cycle; electron transport chains and chemiosmotic phosphorylation mechanism:

After Digestion And Absorption

NS 315 Unit 4: Carbohydrate Metabolism Jeanette Andrade MS,RD,LDN,CDE Kaplan University.

Oxidative decarboxylation of pyruvate and Krebs cycle

Harvesting Electrons from the Citric Acid Cycle

Anti-Importin  3 NIH3T3 cells Primary hippocampal neurons Mouse ES cells Anti-Importin  1 Anti-Importin  1 NIH3T3 cells Primary hippocampal neurons.

Table S4. Summary of proteins identified by MALDI-TOF-MS Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence.

Mitochondrial Electron Transport The cheetah, whose capacity for aerobic metabolism makes it one of the fastest animals.

Fatty acid breakdown The oxidation of fatty acids

Regulation of Cellular respiration and Related pathways.

Supplementary Material 3 Gene ontology annotation of cellular component, molecular function and biological processes for both hypoxia and NAP supplemented.

Citric acid cycle and respiratory chain Pavla Balínová.

The observed and theoretical peptide sequence information Cal.MassObserved. Mass ±da±ppmStart Sequence EndSequenceIon Score C.I%modification FLPVNEK.

Biochemistry ; Lecture TCA & Glyoxylate Cycles There is no reason for this except I love Paris!

3. CITRIC ACID CYCLE. The citric acid cycle (Kreb’s cycle, Tricarboxylic acid cycle) is a series of reactions in mitochondria that bring about the catabolism.

The Citric Acid Cycle & Oxidative phosphorylation.

Oxidative Decarboxylation of pyruvate and TCA cycle

Differential flux analysis of central carbon metabolism. Fluxes which differ by less than 10% between conditions were considered constitutive.

Supplementary Material

Obtaining Energy from Food

September 12 Chapter 24 G&G Fatty acid catabolism

METABOLISM OF LIPIDS: SYNTHESIS OF FATTY ACIDS

Metabolism 1: Catabolism

Pyruvate Oxidation and the Citric Acid Cycle

Lipid-Induced Mitochondrial Stress and Insulin Action in Muscle

Volume 10, Issue 4, Pages (October 2009)

Citric Acid Cycle.

Volume 17, Issue 3, Pages (October 2016)

Comparative Proteomic Profiling of Murine Skin

Proteomic approach in the search of new cardiovascular biomarkers

Volume 7, Issue 3, Pages (May 2014)

Volume 11, Issue 5, Pages (November 2012)

Presentation transcript:

Exacerbated oxidative stress in the fasting liver according to fuel partitioning Thierry Wasselin 1,2, Sandrine Zahn 1,3, Yvon Le Maho 1,3, Alain Van Dorsselaer 1,2, Thierry Raclot 1,3, Fabrice Bertile 1,2,* 1 CNRS, UMR7178, Strasbourg, France 2 Université de Strasbourg, IPHC, Laboratoire de Spectrométrie de Masse Bio-Organique, 25 rue Becquerel, Strasbourg, France 3 Université de Strasbourg, IPHC, Département Ecologie, Physiologie et Ethologie, 23 rue Becquerel, Strasbourg, France * Corresponding Author: Phone : (33) , Fax : (33) , In the following pages, annotated mass spectra are given for all PMF-based identifications. -Peaklists were generated using the Flexanalysis v2.4 software (Bruker Daltonics Gmbh, Bremen, Germany), with the SNAP peak detection algorithm. The signal to noise ratio was set at 4. One cycle of the Savitzky Golay algorithm was used for smoothing. The peaks corresponding to trypsin were removed from the peaklists and spectra annotations. -Mass data were searched using the Mascot algorithm v2.4.1 against the NCBInr database (release ; entries). -Annotated mass spectra are from the Flexanalysis v2.4 software. Intensity coverage values, computed using the BioTools v3.2 software (Bruker Daltonics Gmbh, Bremen, Germany), represent the percentage of matched peak intensities from the whole set of detected peaks in a mass spectrum. This value therefore is an indicator for the amount of nonmatching peaks, but also for the number of intense peaks assigned to the peaklist of the identified protein. As can be seen in the following pages, good results were obtained, with both intensity and sequence coverage values up to 87%. -The number of searched and matched peaks, as well as the sequence of the matched peptides are also given. The m/z ratios of the matched peptides are highlighted in yellow in peaklists. -Mass spectra are given following the order and categorization of Table S2 (Supplementary data). Figure S2. Annotated mass spectra for all mass spectrometry-based identifications

Response to oxidative stress and cell redox homeostasis: Differentially expressed proteins between the fed an fasted states

Spot 47: gi| : carbonic anhydrase 3 [Rattus norvegicus]; Mascot Score: 150 Peaklist Number of mass values searched: 46 Number of mass values matched: 13 Intensity coverage: 78.1 % Sequence coverage: 69.2 % Matched peptide sequences:

Peaklist Spot 84: gi| : Catalase [Rattus norvegicus]; Mascot Score: 314 Matched peptide sequences: Number of mass values searched: 103 Number of mass values matched: 36 Intensity coverage: 68.3% Sequence coverage: 62.8 %

Spot 85: gi| : Catalase [Rattus norvegicus]; Mascot Score: 305 Peaklist Matched peptide sequences: Number of mass values searched: 96 Number of mass values matched: 33 Intensity coverage: 63.4% Sequence coverage: 62.8 %

Spot 86: gi| : Catalase [Rattus norvegicus]; Mascot Score: 392 Peaklist Matched peptide sequences: Number of mass values searched: 80 Number of mass values matched: 39 Intensity coverage: 79.9% Sequence coverage: 63.2 %

Peaklist Matched peptide sequences: Number of mass values searched: 86 Number of mass values matched: 15 Intensity coverage: 64.0% Sequence coverage: 73.0% Peaklist Spot 94: gi| : Carbonic anhydrase 3 [Rattus norvegicus]; Mascot Score: 155

Peaklist Matched peptide sequences: Spot 339 : gi| : Peroxiredoxin 4 [Rattus norvegicus]; Mascot Score: 127 Number of mass values searched: 51 Number of mass values matched: 11 Intensity coverage: 75.1% Sequence coverage: 46.2%

Spot 366 : gi| : mitochondrial aldehyde dehydrogenase [Rattus norvegicus]; Mascot Score: 193 Matched peptide sequences: Peaklist Number of mass values searched: 126 Number of mass values matched: 31 Intensity coverage: 47.3% Sequence coverage: 52.4%

Response to oxidative stress and cell redox homeostasis: Non-differentially expressed proteins between the fed an fasted states

Peaklist Number of mass values searched: 103 Number of mass values matched: 19 Intensity coverage: 21.3% Sequence coverage: 49.0% Matched peptide sequences: Spot 110: gi| : thioredoxin domain containing 4 [Rattus norvegicus]; Mascot Score: 129 Other protein identified in spot 110: Keratin complex 1, matched/total 36/103, Intensity coverage: 52.2%

Peaklist Number of mass values searched: 48 Number of mass values matched: 18 Intensity coverage: 76.5 % Sequence coverage: 85.3 % Matched peptide sequences: Spot 195 : gi| : Peroxiredoxin 6 [Rattus norvegicus]; Mascot Score: 215

Peaklist Number of mass values searched: 58 Number of mass values matched: 18 Intensity coverage: 82.5% Sequence coverage: 67.8% Matched peptide sequences: Spot 197: gi| : Peroxiredoxin 1 [Rattus norvegicus]; Mascot Score: 203

Peaklist Number of mass values searched: 38 Number of mass values matched: 17 Intensity coverage: 86.3% Sequence coverage: 67.8% Matched peptide sequences: Spot 229: gi| : Peroxiredoxin 1 [Rattus norvegicus]; Mascot Score: 242

Peaklist Number of mass values searched: 64 Number of mass values matched: 7 Intensity coverage: 44.5% Sequence coverage: 43.5% Spot 314: gi| : dismutase [Rattus norvegicus]; Mascot Score: 109 Matched peptide sequences:

Peaklist Number of mass values searched: 80 Number of mass values matched: 20 Intensity coverage: 12.5% Sequence coverage: 65% Matched peptide sequences: Spot 316: gi| : glutathione S-transferase, mu 2 [Rattus norvegicus]; Mascot Score: 140 Other protein identified in spot 316: Electron transfer-flavoprotein, matched/total 16/80, Intensity coverage: 30.9%

Protein folding and response to stress: Differentially expressed proteins between the fed an fasted states

Peaklist Number of mass values searched: 104 Number of mass values matched: 58 Intensity coverage: 85.9 % Sequence coverage: 55.2 % Matched peptide sequences: Spot 1 : gi| : endoplasmin [Rattus norvegicus]; GRP94; Mascot Score: 343

Peaklist Matched peptide sequences: Spot 3 : gi| : 84kDa heat shock protein [Rattus norvegicus]; Mascot Score: 292 Number of mass values searched: 114 Number of mass values matched: 47 Intensity coverage: 75.9% Sequence coverage: 54.7%

Matched peptide sequences: Spot 68 : gi| : heat shock 70kD protein 5 [Rattus norvegicus]; GRP78; Mascot Score: 389 Peaklist Number of mass values searched: 110 Number of mass values matched: 48 Intensity coverage: 81.3% Sequence coverage: 60.8%

Peaklist Spot 70 : gi| : Calreticulin [Rattus norvegicus]; Mascot Score: 242 Number of mass values searched: 86 Number of mass values matched: 25 Intensity coverage: 69.3% Sequence coverage: 48.8 % Matched peptide sequences:

Spot 71 : gi| : protein disulfide isomerase [Rattus norvegicus]; Mascot Score: 333 Matched peptide sequences: Peaklist Number of mass values searched: 123 Number of mass values matched: 39 Intensity coverage: 73.0% Sequence coverage: 66.0%

Peaklist Matched peptide sequences: Spot 74: gi| : CaBP1 [Rattus norvegicus]; Mascot Score: 189 Number of mass values searched: 70 Number of mass values matched: 21 Intensity coverage: 67.4 % Sequence coverage: 50.6 %

Protein folding and response to stress: Non-differentially expressed proteins between the fed an fasted states

Peaklist Number of mass values searched: 86 Number of mass values matched: 49 Intensity coverage: 77.7 % Sequence coverage: 64.9% Matched peptide sequences: Spot 64: gi| : stress-70 protein [Rattus norvegicus]; Mascot Score: 372

Matched peptide sequences: Spot 65 : gi| : heat shock cognate 71kDa; heat shock protein 8 [Rattus norvegicus]; Mascot Score: 187 Peaklist Number of mass values searched: 146 Number of mass values matched: 39 Intensity coverage: 35.7% Sequence coverage: 59.9% Other protein identified in spot 65: Annexin A6, matched/total 53/146, Intensity coverage: 37.0%

Peaklist Matched peptide sequences: Number of mass values searched: 114 Number of mass values matched: 51 Intensity coverage: 63.9% Sequence coverage: 67.3% Spot 66 : gi| : heat shock cognate 71kDa; heat shock protein 8 [Rattus norvegicus]; Mascot Score: 369

Peaklist Matched peptide sequences: Peaklist Number of mass values searched: 84 Number of mass values matched: 41 Intensity coverage: 57.7% Sequence coverage: 54.2% Spot 67 : gi| : heat shock cognate 71kDa; heat shock protein 8 [Rattus norvegicus]; Mascot Score: 295

Matched peptide sequences: Spot 75 : gi|56383 : heat shock protein 1 [Rattus norvegicus]; Mascot Score: 281 Peaklist Number of mass values searched: 56 Number of mass values matched: 30 Intensity coverage: 78.8% Sequence coverage: 55.7%

Peaklist Matched peptide sequences: Peaklist Number of mass values searched: 93 Number of mass values matched: 41 Intensity coverage: 78.9 % Sequence coverage: 64.5% Spot 76 : gi|56383 : heat shock protein 1 [Rattus norvegicus]; Mascot Score: 319

Matched peptide sequences: Peaklist Number of mass values searched: 65 Number of mass values matched: 37 Intensity coverage: 69.3 % Sequence coverage: 56.3% Spot 223 : gi| : stress-70 protein [Rattus norvegicus]; Mascot Score: 308

Peaklist Spot 224 : gi| : stress-70 protein [Rattus norvegicus]; Mascot Score: 242 Matched peptide sequences: Peaklist Number of mass values searched: 103 Number of mass values matched: 43 Intensity coverage: 79.8% Sequence coverage: 56.6%

Matched peptide sequences: Peaklist Number of mass values searched: 120 Number of mass values matched: 40 Intensity coverage: 46.2% Sequence coverage: 51.1% Spot 265 : gi| : TNF receptor-associated protein1 [Rattus norvegicus]; Mascot Score: 217

Protein metabolic process / translation: Differentially expressed proteins between the fed an fasted states

Spot 49 : gi| : eukaryotic translation elongation factor 2 [Rattus norvegicus]; Mascot Score: 340 Matched peptide sequences: Peaklist Number of mass values searched: 82 Number of mass values matched: 50 Intensity coverage: 68.6% Sequence coverage: 50.0 %

Spot 102 : gi| : methionine adenosyltransferase I, alpha [Rattus norvegicus]; Mascot Score: 224 Matched peptide sequences: Peaklist Number of mass values searched: 93 Number of mass values matched: 26 Intensity coverage: 82.8% Sequence coverage: 59.6%

Spot 244 : gi| : eukaryotic translation elongation factor 2 [Rattus norvegicus]; Mascot Score: 237 Matched peptide sequences: Peaklist Matched peptide sequences: Number of mass values searched: 90 Number of mass values matched: 47 Intensity coverage: 68.9% Sequence coverage: 43.4%

Spot 249 : gi| : laminin receptor 1 [Rattus norvegicus]; Mascot Score: 143 Matched peptide sequences: Peaklist Number of mass values searched: 87 Number of mass values matched: 19 Intensity coverage: 79.3% Sequence coverage: 53.6%

Spot 350: gi| : threonyl-tRNA synthetase [Rattus norvegicus]; Mascot Score: 155 Matched peptide sequences: Peaklist Number of mass values searched: 68 Number of mass values matched: 26 Intensity coverage: 52.3% Sequence coverage: 31.3%

Protein metabolic process / translation: Non-differentially expressed proteins between the fed an fasted states

Spot 4 : gi| : valosin-containing protein [Rattus norvegicus]; Mascot Score: 416 Matched peptide sequences: Peaklist Number of mass values searched: 117 Number of mass values matched: 61 Intensity coverage: 78.1% Sequence coverage: 60.6%

Spot 5 : gi| : ubiquitin carboxyl-terminal hydroalse 5 [Rattus norvegicus]; Mascot Score: 222 Matched peptide sequences: Peaklist Number of mass values searched: 64 Number of mass values matched: 30 Intensity coverage: 76.5% Sequence coverage: 35.9%

Spot 6 : gi| : ubiquitin carboxyl-terminal hydroalse 5 [Rattus norvegicus]; Mascot Score: 211 Matched peptide sequences: Peaklist Number of mass values searched: 61 Number of mass values matched: 29 Intensity coverage: 73.7% Sequence coverage: 36.0%

Spot 103 : gi| : proteasome 26S subunit, ATPase 2 [Rattus norvegicus]; Mascot Score: 157 Matched peptide sequences: Peaklist Number of mass values searched: 85 Number of mass values matched: 23 Intensity coverage: 22.3% Sequence coverage: 52.7% Other protein identified in spot 103: S-adenosylmethionine synthase, matched/total 18/85, Intensity coverage: 41.8%

Spot 191 : gi| : protease (prosome,macropain) 28 subunit, alpha [Rattus norvegicus]; Mascot Score: 152 Matched peptide sequences: Peaklist Number of mass values searched: 56 Number of mass values matched: 17 Intensity coverage: 42.5% Sequence coverage: 57.8%

Spot 251 : gi| : eukaryotic translation elongation factor 1 alpha 1[Rattus norvegicus]; Mascot Score: 126 Matched peptide sequences: Peaklist Number of mass values searched: 59 Number of mass values matched: 16 Intensity coverage: 57.4% Sequence coverage: 32.8%

Matched peptide sequences: Peaklist Number of mass values searched: 84 Number of mass values matched: 18 Intensity coverage: 50.3% Sequence coverage: 34.4% Spot 257 : gi| : eukaryotic translation elongation factor 1 alpha 1[Rattus norvegicus]; Mascot Score: 115

Spot 363 : gi| : translation elongation factor Tu, mitochondrial precursor [Rattus norvegicus]; Mascot Score: 114 Matched peptide sequences: Peaklist Number of mass values searched: 131 Number of mass values matched: 23 Intensity coverage: 27.8% Sequence coverage: 52.0% Other protein identified in spot 363: Keratin 1, matched/total 26/131, Intensity coverage: 24.7%

Lipid metabolic process: Differentially expressed proteins between the fed an fasted states

Spot 148 : gi| : acyl-CoA synthetase long-chain, member 1 [Rattus norvegicus]; Mascot Score: 420 Matched peptide sequences: Peaklist Number of mass values searched: 98 Number of mass values matched: 47 Intensity coverage: 62.6% Sequence coverage: 56.2%

Spot 181 : gi| : acetyl-CoA dehydrogenase, long-chain, [Rattus norvegicus]; Mascot Score: 197 Matched peptide sequences: Peaklist Number of mass values searched: 83 Number of mass values matched: 31 Intensity coverage: 46.9% Sequence coverage: 54.0% Other protein identified in spot 181: F-alloantigen, matched/total 20/83, Intensity coverage: 26.9%

Spot 202 : gi| : acyl-CoA synthetase long-chain, member 1 [Rattus norvegicus]; Mascot Score: 219 Matched peptide sequences: Peaklist Number of mass values searched: 116 Number of mass values matched: 40 Intensity coverage: 42.8% Sequence coverage: 50.2% Other proteins identified in spot 202: -Keratin 9, matched/total 23/116, Intensity coverage: 18.9% -Keratin 1, matched/total 19/116, Intensity coverage: 13.0%

Spot 266 : gi| : propionyl-CoA carboxylase [Rattus norvegicus]; Mascot Score: 228 Matched peptide sequences: Peaklist Number of mass values searched: 82 Number of mass values matched: 34 Intensity coverage: 59.9% Sequence coverage: 45.1%

Lipid metabolic process: Non-differentially expressed proteins between the fed an fasted states

Spot 88 : gi| : mitochondrial trifunctional protein, alpha subunit [Rattus norvegicus]; Mascot Score: 227 Matched peptide sequences: Peaklist Number of mass values searched: 117 Number of mass values matched: 42 Intensity coverage: 58.7% Sequence coverage: 44.0%

Spot 89 : gi| : mitochondrial trifunctional protein, alpha subunit [Rattus norvegicus]; Mascot Score: 317 Matched peptide sequences: Peaklist Number of mass values searched: 107 Number of mass values matched: 45 Intensity coverage: 72.5% Sequence coverage: 53.9%

Spot 124 : gi| : enoyl Coenzyme A hydratase, short chain 1 [Rattus norvegicus]; Mascot Score: 187 Matched peptide sequences: Peaklist Number of mass values searched: 52 Number of mass values matched: 21 Intensity coverage: 56.8% Sequence coverage: 53.8%

Spot 147 : gi| : carnitine palmitoyltransferase 2 [Rattus norvegicus]; Mascot Score: 109 Matched peptide sequences: Peaklist Number of mass values searched: 78 Number of mass values matched: 20 Intensity coverage: 28.4% Sequence coverage: 28.6% Other protein identified in spot 147: urocanase, matched/total 15/78, Intensity coverage: 34.2%

Matched peptide sequences: Peaklist Number of mass values searched: 81 Number of mass values matched: 31 Intensity coverage: 55.8% Sequence coverage: 42.1% Spot 176 : gi| : carnitine palmitoyltransferase 2 [Rattus norvegicus]; Mascot Score: 223

Matched peptide sequences: Peaklist Number of mass values searched: 92 Number of mass values matched: 21 Intensity coverage: 56.7% Sequence coverage: 55.6% Spot 201 : gi| : enoyl Coenzyme A hydratase, short chain 1 [Rattus norvegicus]; Mascot Score: 154

Matched peptide sequences: Peaklist Number of mass values searched: 80 Number of mass values matched: 26 Intensity coverage: 41.8% Sequence coverage: 37.5% Spot 247 : gi| : carnitine palmitoyltransferase 2 [Rattus norvegicus]; Mascot Score: 188 Other protein identified in spot 247: urocanase, matched/total 25/80, Intensity coverage: 46.4%

Carbohydrate metabolic process: Differentially expressed proteins between the fed an fasted states

Spot 50 : gi| : liver glycogen phosphorylase [Rattus norvegicus]; Mascot Score: 284 Matched peptide sequences: Peaklist Number of mass values searched: 103 Number of mass values matched: 44 Intensity coverage: 59.2% Sequence coverage: 49.1%

Spot 113 : gi| : regucalcin [Rattus norvegicus]; Mascot Score: 191 Matched peptide sequences: Peaklist Number of mass values searched: 108 Number of mass values matched: 31 Intensity coverage: 72.3% Sequence coverage: 71.6%

Spot 137 : gi| : glycerol-3-phosphate dehydrogenase 1 [Rattus norvegicus]; Mascot Score: 259 Matched peptide sequences: Peaklist Number of mass values searched: 61 Number of mass values matched: 26 Intensity coverage: 78.4% Sequence coverage: 59.9%

Spot 355 : gi| : Aldob protein [Rattus norvegicus]; Mascot Score: 205 Matched peptide sequences: Peaklist Number of mass values searched: 48 Number of mass values matched: 20 Intensity coverage: 69.1% Sequence coverage: 43.7%

Carbohydrate metabolic process: Non-differentially expressed proteins between the fed an fasted states

Spot 87 : gi| : transkelotase [Rattus norvegicus]; Mascot Score: 300 Matched peptide sequences: Peaklist Number of mass values searched: 106 Number of mass values matched: 38 Intensity coverage: 69.8% Sequence coverage: 56.8%

Spot 111 : gi| : fructose-1,6-biphosphatase 1 [Rattus norvegicus]; Mascot Score: 212 Matched peptide sequences: Peaklist Number of mass values searched: 95 Number of mass values matched: 27 Intensity coverage: 58.9% Sequence coverage: 57.2%

Spot 170 : gi| : fructose-1,6-biphosphatase 1 [Rattus norvegicus]; Mascot Score: 196 Matched peptide sequences: Peaklist Number of mass values searched: 94 Number of mass values matched: 23 Intensity coverage: 65.3% Sequence coverage: 56.7%

Spot 278 : gi| : ketohexokinase [Rattus norvegicus]; Mascot Score: 116 Matched peptide sequences: Peaklist Number of mass values searched: 57 Number of mass values matched: 14 Intensity coverage: 68.1% Sequence coverage: 49.3%

Spot 308 : gi| : glycerol kinase [Rattus norvegicus]; Mascot Score: 182 Matched peptide sequences: Peaklist Number of mass values searched: 92 Number of mass values matched: 28 Intensity coverage: 51.1% Sequence coverage: 51.7%

Spot 331 : gi| : transkelotase [Rattus norvegicus]; Mascot Score: 206 Matched peptide sequences: Peaklist Number of mass values searched: 156 Number of mass values matched: 33 Intensity coverage: 49.7% Sequence coverage: 49.6%

Spot 333 : gi| : hexose-6-phosphate dehydrogenase [Rattus norvegicus]; Mascot Score: 167 Peaklist Matched peptide sequences: Number of mass values searched: 68 Number of mass values matched: 22 Intensity coverage: 32.7% Sequence coverage: 28.2%

Spot 367 : gi| : galactokinase 1 [Rattus norvegicus]; Mascot Score: 159 Matched peptide sequences: Peaklist Number of mass values searched: 52 Number of mass values matched: 18 Intensity coverage: 47.8% Sequence coverage: 31.4%

Tricarboxylic acid cycle and respiratory electron transport chain : Differentially expressed proteins between the fed an fasted states

Spot 43 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 449 Matched peptide sequences: Peaklist Number of mass values searched: 96 Number of mass values matched: 60 Intensity coverage: 84.8% Sequence coverage: 49.8%

Spot 57 : gi| : succinate dehydrogenase, subunit A [Rattus norvegicus]; Mascot Score: 221 Matched peptide sequences: Peaklist Number of mass values searched: 77 Number of mass values matched: 32 Intensity coverage: 66.0% Sequence coverage: 46.8%

Spot 298 : gi| : dihydrolipoamide dehydrogenase [Rattus norvegicus]; Mascot Score: 123 Matched peptide sequences: Peaklist Number of mass values searched: 87 Number of mass values matched: 17 Intensity coverage: 56.9% Sequence coverage: 40.3%

Tricarboxylic acid cycle and respiratory electron transport chain : Non-differentially expressed proteins between the fed an fasted states

Spot 42 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 350 Matched peptide sequences: Peaklist Number of mass values searched: 73 Number of mass values matched: 47 Intensity coverage: 77.9% Sequence coverage: 35.3%

Spot 44 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 448 Matched peptide sequences: Peaklist Number of mass values searched: 149 Number of mass values matched: 77 Intensity coverage: 75.0% Sequence coverage: 60.3%

Spot 45 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 407 Matched peptide sequences: Peaklist Number of mass values searched: 85 Number of mass values matched: 53 Intensity coverage: 76.7% Sequence coverage: 43.5%

Spot 72 : gi| : ATP synthase, F1 complex, beta subunit [Rattus norvegicus]; Mascot Score: 331 Matched peptide sequences: Peaklist Number of mass values searched: 109 Number of mass values matched: 36 Intensity coverage: 75.2% Sequence coverage: 87.3%

Spot 107 : gi| : ubiquinol-cytochrome c reductase core protein 1 [Rattus norvegicus]; Mascot Score: 100 Matched peptide sequences: Peaklist Number of mass values searched: 105 Number of mass values matched: 20 Intensity coverage: 43.5% Sequence coverage: 40.4% Other protein identified in spot 107: keratin, matched/total 31/105, Intensity coverage: 34.5%

Spot 112 : gi| : Suclg2 protein [Rattus norvegicus]; Mascot Score: 321 Matched peptide sequences: Peaklist Number of mass values searched: 76 Number of mass values matched: 34 Intensity coverage: 71.0% Sequence coverage: 56.6%

Spot 117 : gi| : isocitrate dehydrogenase 1 [Rattus norvegicus]; Mascot Score: 341 Matched peptide sequences: Peaklist Number of mass values searched: 127 Number of mass values matched: 42 Intensity coverage: 73.4% Sequence coverage: 69.3%

Spot 159 : gi| : dehydrogenase E1 and transkelotase domain 1 [Rattus norvegicus]; Mascot Score: 119 Matched peptide sequences: Peaklist Number of mass values searched: 72 Number of mass values matched: 23 Intensity coverage: 44.9% Sequence coverage: 20.9%

Spot 160 : gi| : dehydrogenase E1 and transkelotase domain 1 [Rattus norvegicus]; Mascot Score: 156 Matched peptide sequences: Peaklist Number of mass values searched: 49 Number of mass values matched: 22 Intensity coverage: 46.4% Sequence coverage: 21.6%

Spot 174 : gi| : rCG55067 (aconitase 1) [Rattus norvegicus]; Mascot Score: 431 Matched peptide sequences: Peaklist Number of mass values searched: 115 Number of mass values matched: 64 Intensity coverage: 79.1% Sequence coverage: 59.4%

Spot 175 : gi| : aconitase 2 [Rattus norvegicus]; Mascot Score: 296 Matched peptide sequences: Peaklist Number of mass values searched: 70 Number of mass values matched: 35 Intensity coverage: 75.8% Sequence coverage: 45.0%

Spot 205 : gi| : aconitase 2 [Rattus norvegicus]; Mascot Score: 250 Matched peptide sequences: Peaklist Number of mass values searched: 75 Number of mass values matched: 31 Intensity coverage: 67.6% Sequence coverage: 41.8%

Spot 208 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 321 Matched peptide sequences: Peaklist Number of mass values searched: 65 Number of mass values matched: 40 Intensity coverage: 71.6% Sequence coverage: 34.0%

Spot 209 : gi| : pyruvate carboxylase [Rattus norvegicus]; Mascot Score: 401 Matched peptide sequences: Peaklist Number of mass values searched: 125 Number of mass values matched: 65 Intensity coverage: 64.8% Sequence coverage: 48.2%

Spot 270 : gi| : ATP synthase, F1 complex, alpha subunit [Rattus norvegicus]; Mascot Score: 338 Matched peptide sequences: Peaklist Number of mass values searched: 81 Number of mass values matched: 40 Intensity coverage: 70.8% Sequence coverage: 61.9%

Spot 271 : gi| : ATP synthase, F1 complex, alpha subunit [Rattus norvegicus]; Mascot Score: 106 Matched peptide sequences: Peaklist Number of mass values searched: 131 Number of mass values matched: 24 Intensity coverage: 26.6% Sequence coverage: 37.9%

Spot 290 : gi| : NADH dehydrogenase (ubiquinone) Fe-S protein 1 [Rattus norvegicus]; Mascot Score: 267 Matched peptide sequences: Peaklist Number of mass values searched: 62 Number of mass values matched: 35 Intensity coverage: 81.0% Sequence coverage: 44.3%

Spot 327 : gi| : succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial [Rattus norvegicus]; Mascot Score: 241 Matched peptide sequences: Peaklist Number of mass values searched: 55 Number of mass values matched: 31 Intensity coverage: 79.8% Sequence coverage: 51.4%

Spot 332 : gi| : aconitase 2 [Rattus norvegicus]; Mascot Score: 223 Matched peptide sequences: Peaklist Number of mass values searched: 35 Number of mass values matched: 23 Intensity coverage: 77.6% Sequence coverage: 30.4%

Other: Differentially expressed proteins between the fed an fasted states

Spot 52 : gi| : dimethylglycine dehydrogenase precursor [Rattus norvegicus]; Mascot Score: 312 Matched peptide sequences: Peaklist Number of mass values searched: 98 Number of mass values matched: 44 Intensity coverage: 69.6% Sequence coverage: 49.5%

Spot 68 : gi| : catechol-O-methyltransferase [Rattus norvegicus]; Mascot Score: 157 Matched peptide sequences: Peaklist Number of mass values searched: 65 Number of mass values matched: 19 Intensity coverage: 69.3% Sequence coverage: 63%

Spot 80 : gi| : transferrin [Rattus norvegicus]; Mascot Score: 404 Matched peptide sequences: Peaklist Number of mass values searched: 89 Number of mass values matched: 42 Intensity coverage: 84.6% Sequence coverage: 56.4%

Spot 81 : gi| : transferrin [Rattus norvegicus]; Mascot Score: 366 Matched peptide sequences: Peaklist Number of mass values searched: 65 Number of mass values matched: 38 Intensity coverage: 83.2% Sequence coverage: 52.7%

Spot 82 : gi| : transferrin [Rattus norvegicus]; Mascot Score: 370 Matched peptide sequences: Peaklist Number of mass values searched: 64 Number of mass values matched: 39 Intensity coverage: 83.3% Sequence coverage: 54.6%

Spot 84 : gi| : delta-1-pyrroline-5-carboxylate dehydrogeanse [Rattus norvegicus]; Mascot Score: 261 Matched peptide sequences: Peaklist Number of mass values searched: 75 Number of mass values matched: 27 Intensity coverage: 67.1% Sequence coverage: 53.4%

Spot 93 : gi| : methylmalonate-semialdehyde dehydrogenase [Rattus norvegicus]; Mascot Score: 208 Matched peptide sequences: Peaklist Number of mass values searched: 108 Number of mass values matched: 32 Intensity coverage: 66.3% Sequence coverage: 48.0%

Spot 121 : gi| : 3-alpha-hydroxysteroid dehydrogenase [Rattus norvegicus]; Mascot Score: 301 Matched peptide sequences: Peaklist Number of mass values searched: 52 Number of mass values matched: 24 Intensity coverage: 82.6% Sequence coverage: 67.7%

Spot 122 : gi| : 3-alpha-hydroxysteroid dehydrogenase [Rattus norvegicus]; Mascot Score: 114 Matched peptide sequences: Peaklist Other protein identified in spot 122: methyltransferase, matched/total 18/113, Intensity coverage: 55.0% Number of mass values searched: 113 Number of mass values matched: 20 Intensity coverage: 11.5% Sequence coverage: 56.8%

Spot 122 : gi| : glycine N-methyltransferase [Rattus norvegicus]; Mascot Score: 149 Matched peptide sequences: Peaklist Number of mass values searched: 99 Number of mass values matched: 22 Intensity coverage: 55.4% Sequence coverage: 60.6%

Spot 140 : gi| : sarcosine dehydrogenase [Rattus norvegicus]; Mascot Score: 367 Matched peptide sequences: Peaklist Number of mass values searched: 79 Number of mass values matched: 43 Intensity coverage: 87.2% Sequence coverage: 38.8%

Spot 150 : gi| : catechol-O-methyltransferase [Rattus norvegicus]; Mascot Score: 160 Matched peptide sequences: Peaklist Number of mass values searched: 59 Number of mass values matched: 19 Intensity coverage: 74.0% Sequence coverage: 59.0%

Spot 192 : gi| : estrogen sulfotransferase[Rattus norvegicus]; Mascot Score: 233 Matched peptide sequences: Peaklist Number of mass values searched: 92 Number of mass values matched: 31 Intensity coverage: 59,1% Sequence coverage: 65.4%

Spot 193 : gi| : guanidoacetate methyltransferase [Rattus norvegicus]; Mascot Score: 131 Matched peptide sequences: Peaklist Number of mass values searched: 64 Number of mass values matched: 16 Intensity coverage: 52.0% Sequence coverage: 67.2%

Spot 196 : gi| : nitrilase family member 2 [Rattus norvegicus]; Mascot Score: 238 Matched peptide sequences: Peaklist Number of mass values searched: 49 Number of mass values matched: 20 Intensity coverage: 79.8% Sequence coverage: 65.6%

Spot 202 : gi| : methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial [Rattus norvegicus]; Mascot Score: 159 Matched peptide sequences: Peaklist Number of mass values searched: 48 Number of mass values matched: 21 Intensity coverage: 53.7% Sequence coverage: 26.0%

Spot 214 : gi| : purine nucleoside phosphorylase [Rattus norvegicus]; Mascot Score: 219 Matched peptide sequences: Peaklist Number of mass values searched: 70 Number of mass values matched: 24 Intensity coverage: 70.1% Sequence coverage: 66.8%

Spot 215 : gi| : Sulfotransferase 1C1 [Rattus norvegicus]; Mascot Score: 171 Matched peptide sequences: Peaklist Number of mass values searched: 91 Number of mass values matched: 27 Intensity coverage: 66.0% Sequence coverage: 55.3%

Spot 241 : gi| : transferrin [Rattus norvegicus]; Mascot Score: 395 Matched peptide sequences: Peaklist Number of mass values searched: 89 Number of mass values matched: 45 Intensity coverage: 81.9% Sequence coverage: 58.2%

Spot 325 : gi| : branched chain ketoacid dehydrogenase E1 beta [Rattus norvegicus]; Mascot Score: 132 Matched peptide sequences: Peaklist Number of mass values searched: 32 Number of mass values matched: 12 Intensity coverage: 64.6% Sequence coverage: 31.8%

Spot 344 : gi| : Ppa1 protein [Rattus norvegicus]; Mascot Score: 118 Matched peptide sequences: Peaklist Number of mass values searched: 73 Number of mass values matched: 18 Intensity coverage: 66.3% Sequence coverage: 48.7%

Spot 364 : gi| : SEC23A [Rattus norvegicus]; Mascot Score: 113 Matched peptide sequences: Peaklist Number of mass values searched: 99 Number of mass values matched: 26 Intensity coverage: 33.2% Sequence coverage: 29.4% Other proteins identified in spot 364: -keratin9, matched/total 21/99, Intensity coverage: 20.6% -keratin1, matched/total 20/99, Intensity coverage: 20.9%