The microbial world S. Cerevisiae (yeast) Mycobacterium tuberculosis.

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Presentation transcript:

The microbial world S. Cerevisiae (yeast) Mycobacterium tuberculosis

Metagenomics Diversity Analysis 16sRNA sequencing Fingerprinting Hybridization Last week: Extracted DNA Amplified 16s rRNA gene This week: Visualize PCR product (gel electrophoresis) Functional analysis for antibiotic resistance

DNA Primers MgCl 2 Buffer dNTPs Taq DNA polymerase PCR mixture requirements

Metagenomics Gel Electrophoresis

Metagenomics Functional Analysis Are there genes producing proteins that lead to antibiotic resistance?

Metagenomics Similar to Kirby-Bauer disk diffusion susceptibility test - standardized protocol to measure resistance or sensitivity of pathogens to potential antibiotic compounds. Bacteria lawn Disc containing antibiotic Clearing zone

Metagenomics Factors affecting size of clearing zone: Effectiveness of antibiotic Concentration of antibiotic Solubility of antibiotic Depth of agar Density of bacteria Resistant colonies –things to consider Distance from disc Number