Biotech Equations. Preparing Solutions What is a solution? – Mixture of two ore more substances Solutions have two parts – Solute – substance that is.

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Presentation transcript:

Biotech Equations

Preparing Solutions What is a solution? – Mixture of two ore more substances Solutions have two parts – Solute – substance that is being dissolved – Solvent – substance doing the dissolving

Making Mass/Volume Solutions ____ g/mL X ____ mL = ____ g of solution concentrationvolumeto be weighed out, desireddesireddissolved in the solvent

What mass of the protein, gelatin, is needed to make 0.5 L of a 3 g/L gelatin solution?

Mass/Volume Concentration Equation ____ % = ____ percent valuedecimal value of the g/mL ____ X ____ = ____ g of solute to decimaltotal volumebe measured and valuedesired (mL)added to the volume desired of solvent

What mass of gelatin is needed to make 5 L of a 6% gelatin solution?

Molar mass of a substance – Find by using the periodic table! – The masses on the periodic table are of one mole of that substance – Mole – unit of measurement used to express the amount of a chemical substance

Find the molar mass of – NaCl – O 2 – HCl – CH 3 COOH

Find the molar mass of – NaCl  – O 2  – HCl  – CH 3 COOH  g/mol 32 g/mol g/mol g/mol

End for today

What if we are given grams of a substance…. Not moles?! If this happens we will have to do stoichiometry Going from grams of a substance to moles of that same substance How many moles will I have if I have 3 grams of Hydrogen?

Can also go the other way… I’m given moles and I need grams!! Convert to grams 2 mol sodium chloride

Molarity (M)

What is the molarity of a solution that contains 4.53 moles of lithium nitrate in 2.85 liters of solution?