Using BLAST and ClustalW2 for Multiple Sequence Alignment IBGP/BMI705 Lab 2 January 14, 2009 Lee Cooper, TA

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Presentation transcript:

Using BLAST and ClustalW2 for Multiple Sequence Alignment IBGP/BMI705 Lab 2 January 14, 2009 Lee Cooper, TA

Have you ever used bioinformatics tools?

Why should I care about understanding bioinformatics? Applicable Focus benchwork Preliminary data Free, readily-available tools vast amounts of information powerful Required knowledge in 5-10 years Career marketability

Summary: BLAST/ Sequence Alignment A—Search genes (5 minutes) B—BLAST (10 minutes) C—Pair BLAST (10 minutes) D—ClustalW2 (10 minutes)

Today’s guided lab: BLAST/ Sequence Alignment Follow handout Ask questions Work together Answer questions short-answer format on paper turn in to TA today (Pass/fail) Next week’s lab– SAME handout and tools Practice outside of class How can you use these tools to help along your current research and/or studies?

A. Search for gene/s

B. BLAST

C. Pair BLAST: Comparing Homo sapiens E2F3 to Mus musculus E2f3

D. ClustalW2 Sequence Alignment

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