Laboratory of bio cybernetics

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Presentation transcript:

Laboratory of bio cybernetics http://lbk.fe.uni-lj.si Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic, Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek, David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl, Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic. (Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)

Applications of Electroporation Exposure to electric field irreversible Cell death + E - reversible Introducing small molecules Extraction of molecules Introducing large molecules EP Cell fusion Protein insertion in the plasma membrane

ECT day 0 10 weeks after ECT selectivity of ECT Julie Gehl. Ugeskrift for Laeger 167(34): 3156-9, 2005. Jarm, Cemazar, Miklavcic, Sersa. Expert Rev. Anticancer Ther. 10: 729–746, 2010

Mode of operation in Laboratory of Biocybernetics

Capacities and experties 2 cell culture labs 1 cell culture lab safety level 2 1 microbiology cell lab Skin ex vivo lab; lipid bilayer Electronic workshop Impedance and temperature measurements Modelling (biological systems, electronic circuitry, multiphysics, multiscale…) Cell electroporation Lipid bilayer Vesicles (GUV, SUV,…) Cells (attached, suspension, in matrigel…) Cells: single, clusteres Non-excitable, excitable Skin ex vivo Extraction of intracellular components from cells Dielectrophoresis for cell separation Bioimpedance measurement; electrical characterisation

See also: www.electroporation.net Damijan Miklavčič University of Ljubljana Faculty of Electrical Engineering Ljubljana, SLOVENIA http://lbk.fe.uni-lj.si

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