Cry3A transgenic, bark beetle resistant spruce NAZV project QH71290 2007-2011.

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Presentation transcript:

Cry3A transgenic, bark beetle resistant spruce NAZV project QH

Spruce bark beetle (Ips typographus)

Bark beetle galleries

Bacillus thuringiensis crystal protein (Cry, δ-endoTOXin)

Agrobacterium interaction with the plant cell

Spruce is difficult to transformSpruce is difficult to transform No known Bt toxin is toxic enough to bark beetleNo known Bt toxin is toxic enough to bark beetle Major problems:

Spruce callus cultures

Somatic embryogenesis induction conversion cultivation

Modified Cry3A genes Cry3A orig Synt TOX-1 (602 aa)-codons Picea optimized Ndel/sTOX-1 Cdel/sTOX-1 (602 aa) Cdel/Ndel/sTOX-1GUS

Refined modification of domain II

Expression vector

PAGE analysis of modified Cry3A expression

WESTERN detection of modified Cry3A proteins

Toxicity tests on live beetles

K o n e c p r e s e n t a c e

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