Snapshot of iron response in Shewanella oneidensis by gene network reconstruction Emily Simso and Ronald Legaspi Departments of Biology and Computer Science.

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Snapshot of iron response in Shewanella oneidensis by gene network reconstruction Emily Simso and Ronald Legaspi Departments of Biology and Computer Science Loyola Marymount University November 17, 2015

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Defining necessary vocabulary Iron chelator Induced Repressed Repletion Depletion Up-regulated Down-regulated Aerobic Anaerobic

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

The significance of Shewanella oneidensis as a model organism -S. oneidensis is a pathogen to both fish and humans -Iron-regulated metabolisms are often needed for virulent organisms  Vibrio cholerae  Bacillus cereus -Could be used to remediate U.S. Department of Energy’s uranium-contaminated sites due to grouping of transcriptional regulators

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Experimental design for the study

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Studying iron depletion in an anaerobic state

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Three major modules in gene co-expression network

Overview Defining necessary vocabulary The significance of Shewanella oneidensis as a model organism Experimental design for the study Studying iron depletion in an anaerobic state Analyzing results through figures and tables Discussion and significance of results, contextualized with other organisms

Anaerobic energy metabolism model New role of Crp Lack of oxidative stress Fur-independent results Rapid uptake of iron in S. oneidensis

References Biology Online. N.p., 27 May Web. 15 Nov Yang, Y., Harris, D. P., Luo, F., Xiong, W., Joachimiak, M., Wu, L., … Zhou, J. (2009). Snapshot of iron response in Shewanella oneidensis by gene network reconstruction. BMC Genomics, 10,

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