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Supplemental Table 1. Phenotypes in sleepy1 mutants. Fertility (number of seeds/one silique) Stem (cm) (after 60 days) Leaf (color) sly1-t2 (WS)dark green19.7.

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Presentation on theme: "Supplemental Table 1. Phenotypes in sleepy1 mutants. Fertility (number of seeds/one silique) Stem (cm) (after 60 days) Leaf (color) sly1-t2 (WS)dark green19.7."— Presentation transcript:

1 Supplemental Table 1. Phenotypes in sleepy1 mutants. Fertility (number of seeds/one silique) Stem (cm) (after 60 days) Leaf (color) sly1-t2 (WS)dark green19.7 + 1.9 (40.5%)14.5 + 2.2 (26.0%) WT (WS)55.7 + 1.248.7 + 2.2green WT (Col)47.8 + 1.4green51.4 + 2.4 WT (Ler)36.4 + 1.4green61.3 + 2.9 sly1-2 (Ler)dark green5.1 + 0.4 (14.0%)13.6 + 1.2 (22.2%) sly1-10 (Ler) dark green10.9 + 0.5 (22.8%)16.4 + 1.0 (26.8%) Line (background) % shows the relative rate in sly1 mutant compared to the wild type.

2 100 80 60 40 20 0 #35#37#40#41#42 Germination (%) Seed stock lot 0.5 month 1.0 month 8 months Supplemental Figure 1. Ariizumi and Steber

3 0 % PEG 4 % PEG 6 % PEG 8 % PEG 100 80 60 40 20 0 Germination (%) A 0 % PEG 4 % PEG 6 % PEG 8 % PEG 100 80 60 40 20 0 Germination (%) B 0 % PEG 4 % PEG 6 % PEG 8 % PEG 01234567 100 80 60 40 20 0 Germination (%) Time (days) C Supplemental Figure 2. Ariizumi and Steber

4 Ler (WT) (30) ga1-3 (27) sly1-10 (29) sly1-2 (A) (28) N.G. 100 80 60 40 20 0 Germination (%) 0.1 µM GA 3 1.0 µM GA 3 10 µM GA 3 0 µM GA 3 Supplemental Figure 3. Ariizumi and Steber

5 B WT (Ler) sly1-10 sly1-2 (A) sly1-2 (D) sly1-2 rgl2-13 ga1-3 ga1-3 rga-t2 WT (Ws) sly1-t2 RGL2 (60.5 KDa) Age (month) sly1-10 sly1-2 271540.5 26 RGL2 (60.5 KDa) 60626316 D Supplemental Figure 4. Ariizumi and Steber C Ler (WT) Ws (WT) 6531Dry 1 2 4oC4oC22 o C * * * * RGL2 (60.5 KDa) rgl2-13 RGL2 (60.5 KDa) * * A ga1-3 rgl2 4 o C 6 d 22 o C 6 d 60.5 KDa * * Days

6 Ler (WT) ga1-3 sly1-10 sly1-2 (A) sly1-2 (D) Dry135 6 12345 6 4oC4oC 22 o C after 3 d stratification 100 80 60 40 20 0 0123456 Radicle emergence (%) Time (days) Ler (WT) sly1-10 sly1-2 (A) sly1-2 (D) ga1-3 Supplemental Figure 5. Ariizumi and Steber sly1-2 rgl2-13 sly1-2 (A) sly1-2 gai-t6 sly1-2 rga24 ga1-3 RGA (64.0 KDa) RGL2 Non specific Days sly1-2 rga24 Dry 4oC4oC 22 o C 4oC4oC sly1-10 ga1-3 rga-t2 6 155 6 6 RGA (64.0 KDa) Non specific A B C D Days

7 A B WT (Ler) rgl2-13 (Col) Negative WT (Col) WT (Ws) RGL2 ACTIN2 Supplemental Figure 6. Ariizumi and Steber

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