1. If = Hypothesis, Then = Prediction Prediction= What you think the outcome of a specific experiment will be Hypothesis: Why If /then statement is NOT A HYPOTHESIS You will be marked wrong.

2. You Should Know Scientific Method Science Terms – Control – Controlled Variable, – Indpendent/dependent variables – Sample size – Significance – Etc…. Tools we have learned in lab. From onions- transformations. Big picture.

3. Hint: We are testing Scientific reasoning and problem soloving.

4. Transformations Terms – Biotechnology: using technology to manipulate organisms – Genetic Engineering: Manipulating genes in organisms – DNA Cloning: putting a segment of DNA in an organism and having that organism make tons of copies of the gene, gene product or DNA segment. – Transformation: Introducing DNA from one organism (usually bacteria) into another organism.

5. Very Expensive 2 stops Will be Graded on Technique Be patient

6. Restriction Enzymes Leave Sticky Ends

9. Transformation http://www.youtube.com/watch?v=aA5fyWJh5S0

10. How do we know it worked? Must determine if Gene was Inserted Successfully into Plasmid – Marker Genes! – GFP – Disrupt function and it disappears Antibiotic: tells if plasmid made it in to cell Color/Glowing: tells if gene made it into plasmid

13. Problem

14. Plates Groups of 3 or 4. Half Plates: step 7 page 104 use half volumes – 50 instead of 100, 25 instead of 50 Check Plates 48 hours later mark on plate cover what you found. – Leave in incubator for me to grade.