1. Lab 26 Goals and Objectives: Same FOUR GROUPS for all activities Exercise 28: Temperature Effects on Growth Mix cultures well with pipette and transfer to cuvettes for O.D. reading (absorbance): record data on class sheet, Record combined data Exercise 29: pH and Microbial Growth Mix cultures well with pipette and transfer to cuvettes for O.D. reading (absorbance): record data on class sheet, Record combined data Exercise 62: Temperature Lethal Effects Score plates + or - for growth using control for comparison: record data on class sheet, Record combined data Exercise 31: Lethal Effect of UV Light Do your assigned organism and times (see assignment cards) Completely cover plate with organism: swab three times Draw a line to indicate boundary of the card Lid off, agar side up under lamp (unless assigned “with lid”) Exercise 32: Effects of Lysozyme on Bacterial Cells Observe and record provided results **Bring favorite disinfectant/antimicrobial to next lab to test!

2. TempO.D. 4°C0.03 25°C0.51 37°C0.72 42°C0.63 55°C0.14

3. ++++ +++- +--- ---- TDP? TDT @70°C? @80°C? @90°C? @100°C? 62

4. Plates agar side up, no lid, cover half with paper card Cover half with paper card Draw line on plate to indicate boundary of card UV Exposed side Control (was covered) Exercise 31

5. Lab 26 Goals and Objectives: Same FOUR GROUPS for all activities Exercise 28: Temperature Effects on Growth Mix cultures well with pipette and transfer to cuvettes for O.D. reading (absorbance): record data on class sheet, Record combined data Exercise 29: pH and Microbial Growth Mix cultures well with pipette and transfer to cuvettes for O.D. reading (absorbance): record data on class sheet, Record combined data Exercise 62: Temperature Lethal Effects Score plates + or - for growth using control for comparison: record data on class sheet, Record combined data Exercise 31: Lethal Effect of UV Light Do your assigned organism and times (see assignment cards) Completely cover plate with organism: swab three times Draw a line to indicate boundary of the card Lid off, agar side up under lamp (unless assigned “with lid”) Exercise 32: Effects of Lysozyme on Bacterial Cells Observe and record provided results **Bring favorite disinfectant/antimicrobial to next lab to test!