1. Phylogeographic structure of Tridacna crocea across the Indo West Pacific “How diverse is your clam chowda?” Matthew Subia 1, Paul Barber 2 1 CSU Monterey Bay, 2 Boston University

2. Fish Corals Snails Roberts et al. 2002 IWP is Center of Marine Biodiversity

3. Giant Clam Distribution

5. Giant Clam Importance Food Colorful decorations During 1960’s and 70’s annual landings estimated at 100-400 tons. In 1985, clams were included in the convention on International Trade in Endangered Species of Wild Fauna and flora

7. Concerns for Giant Clams Commercial and artisanal exploitation Bleaching Disease Pollution Poaching

8. Tridacna crocea

9. Natural History of T. crocea Adults tend to burrow in coral in waters less than 10 m deep Hermaphroditic and reach sexual maturity in approximately 5-7 years Broadcast spawners –Sperm released first –Eggs follow Algal symbionts and filter feeders

10. Pacific Ocean Indian Ocean Australia Asia 400km

11. Coastal margins during low sea levels stands Indonesia in the Pleistocene After Voris 2002 400km

12. Williams et al. 2002 Genetic differentiation of Pacific and Indian Ocean populations of Starfish

13. Lavery et al. 1996 McMillan & Palumbi 1996 Williams & Benzie, Benzie & Williams 1997 Duke et al. 1999

14.  ST =0.87 p=0 H. pulchella

16. Sampled C & W Pacific Populations

17. Plotted Nm values on geography

18. Objectives What are the patterns of genetic structuring of T. crocea in Indonesia? Is there evidence for limited genetic exchange among populations? Do they show Pacific-Indian Ocean split?

19. Methods Extracted DNA from T. crocea tissue samples using Chelex Amplified DNA using PCR and mtCO1 T. crocea specific primers Sequenced DNA using ABI 377 Sequences aligned and edited using Sequencher 4.5 Sequences analyzed in Arlequin 2.0

20. N=287

21. Geographic Distribution

22. AMOVA Results

23. Pairwise Comparison

24. Conclusion T. crocea demonstrates a new pattern in comparison to previously studied species in this region Three distinct clades exist: –1 in the far west –1 in the far east –1 in central Therefore, T. crocea shows regional genetic structure

25. Acknowledgements Paul Barber Elizabeth Jones Joshua Drew Eric Crandall Timery DeBoer Craig Starger NSF