1. SiLENT DO NOW Read the lab when you are done  Explain how the solute will move according to diffusion.  Describe what the cells will look like after diffusion. (Red = soluteWhite = solvent) CELL ‘A’ CELL ‘B’

2. Agenda  Catalyst (7 minutes)  Diffusion Blitz Review (5 minutes)  OSMOSIS! (7 minutes)  Guided Practice (25 minutes)  Independent Practice (21 minutes)  Egg Lab (25 minutes)  EQ (10 minutes) HOMEWORK: COMPLETE WORKSHEET

3. Diffusion  Key Point #1: Diffusion is the net movement of molecules from an area of high concentration to an area of low concentration  Key Point #2: Diffusion continues until there is equilibrium  Key Point #3: Cell membranes are selectively permeable

4. Infrared Fart ON YOUR OWN, IN YOUR NOTES…  Using the process of diffusion, explain to your friend why you can smell their fart even though they are sitting on the other side of the room.

5. OBJECTIVES By the end of the period, all SWBAT…  Predict the movement of water using osmosis

6. Osmosis!  Key Point #1: Osmosis is the diffusion of water  Special kind of diffusion  The solvent (water) moves, instead of the solute (stuff)  But… why?!?!

7. Blue=water, Green=solute

9. So What?  Key Point #2: When cells are placed in hypertonic or hypotonic solutions, they change in volume  Hyper- = high  Hypo- = low  Iso- = same

10. Hypertonic Solution Outside Cell Inside Cell 70% water 50% water

11. Water Hypertonic Solution Outside Cell Inside Cell 70% water 50% water

12. Hypertonic Solution Water Inside Cell Outside Cell 60% water

13. Hypotonic Solution Outside Cell Inside Cell 22% water 40% water

14. Water Hypotonic Solution Outside Cell Inside Cell 22% water 40% water

15. Inside Cell Water Hypotonic Solution Outside Cell 30% water

16. Isotonic Solution Inside Cell Outside Cell 90% water

17. Red blood cells in action  http://www.coolschool.ca/lor/BI12/unit4/U04L06/ rbc.html http://www.coolschool.ca/lor/BI12/unit4/U04L06/ rbc.html

18. Whiteboards!Goal: 60s Pull into groups and get your whiteboards, please!

19. Inside Cell Outside Cell 16% water 7% water

20. Outside Cell Inside Cell 54% water 88% water

21. Inside Cell Outside Cell 1% water

22. Hypo, hyper or isotonic? 40% water inside the cell and 10% water outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

23. Hypo, hyper or isotonic? 10% water inside the cell and 90% water outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

24. Hypo, hyper or isotonic? 55% water inside the cell and 55% water outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

25. PAY ATTENTION!!!  20% SUGAR inside the cell and 22% SUGAR outside the cell. The cell membrane is permeable to water.

26. Hypo, hyper or isotonic? 20% sugar inside the cell and 22% sugar outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

27. Hypo, hyper or isotonic? 80% sugar inside the cell and 80% sugar outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

28. Hypo, hyper or isotonic? 9% sugar inside the cell and 1% sugar outside the cell. The cell membrane is permeable to water. 1)Is this hypertonic, hypotonic, or isotonic? 2)How will the water move, according to osmosis? 3)What will happen to the cell?

29. We’re getting tricky!  Ocean water has a higher concentration of salt than do human body cells. Imagine that poor Darius is stranded out at sea, and very thirsty. He drinks a bunch of seawater.  What type of solution is ocean water (hypertonic, hypotonic, isotonic)?  What will happen to his cells as a result?

30. We’re getting tricky!  Ocean water has a lower concentration of water than do human body cells.  What type of solution is ocean water (hypertonic, hypotonic, isotonic)?  What would happen to your cells if you drank this ocean water?

31. We’re getting tricky!  Distilled water has a higher concentration of water than do human body cells.  What type of solution is distilled water (hypertonic, hypotonic, isotonic)?  What would happen to your cells if you drank distilled water?

32. We’re getting tricky!  Distilled water has a lower concentration of minerals than do human body cells.  What type of solution is distilled water (hypertonic, hypotonic, isotonic)?  What would happen to your cells if you took a bath in distilled water?

33. Worksheet  Everyone loves… WORKSHEETS!!!

34. Egg Lab – Day 1  An egg can be thought of as a big cell. After the shell is removed (with vinegar), it is a partially permeable membrane.  Putting this egg in a hyper/hypotonic solution will cause osmosis to occur!

35. Egg Lab – Day 1 QUESTION: Will an unshelled egg increase or decrease in mass after being placed overnight in corn syrup or water?

36. Egg Lab – Day 1 HYPOTHESIS: Formulate two hypotheses, one for putting an egg in water, the other for putting an egg in corn syrup. (Formulate means “create.”) Example: 1. If the egg is placed in water, then it will increase/decrease in mass because ____________. 2. If the egg is placed in corn syrup, then it will increase/decrease in mass because ____________. CHOOSE ONE

37. Egg Lab – Day 1 VARIABLES:  Identify your INDEPENDENT and DEPENDENT variables. Remember: Categories!!!  Identify at least 2 CONSTANTS. REMEMBER: Variables are categories, not specific things!

38. Egg Lab – Day 1 SET UP: 1. Observe each egg. Record your observations in Data Table 2. 2. Calculate the mass of each egg. Record this mass in Data Table 1. 3. Label one beaker A and one beaker B. Put one egg in each. 4. Add enough acetic acid to the beaker so that you are completely covering the egg. 5. In Data Table 2, record detailed observations of the eggs.

39. How do you use a triple-beam balance?  Calibrate using knob on the left! Make sure balance is pointing at 0 before you start.  Put object on metal balance.  Start with the highest beam (100 g) increments. Move it over one by one notch until the balance goes below 0. Do this for each beam until you have you balance at 0.

40. What if we want to take the mass of an egg?  It might roll off and die!  Correct method:  Take mass of the beaker. (WRITE IT DOWN – Data Table 1)  Take the mass of the beaker and the egg inside it.  Subtract the first mass from the 2 nd to get the mass of the egg. Mass of Beaker+Egg – Mass of Beaker = Mass of Egg

41. Exit Question 1. A cell contains a solution that is 44% water, and it is sitting in a solution that is 70% water. 2. A cell contains a solution that is 64% water, and it is sitting in a solution that is 64% water. 3. A cell contains a solution that is 9% sugar, and it is sitting in a solution that is 88% sugar.  Identify the type of solution  Describe what will move, and where  Explain how the cell’s volume will change  Identify the type of solution  Describe what will move, and where  Explain how the cell’s volume will change