1. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman Science 7 November 2014 vol. 346 p716 1257521-1

4. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 1. A general aproach for subcellular ribosome profiling: development and application to the ER. Science 7 November 2014 vol. 346 p716 1257521-1

5. 1. A general aproach for subcellular ribosome profiling: development and application to the ER.

7. Calvin Jan et al. (2014) Figure 1a.

8. 1. Ribosome isolation: sedimentation centrifugation.

11. 1. Western Blot with Immunostaining.

14. Calvin Jan et al. (2014) Figure 1b

15. Calvin Jan et al. (2014) Figure S1

16. Biotin

17. Perinuclear ER

18. Calvin Jan et al. (2014) Figure 1c

19. 1. Fine-tunig the assay:

20. Calvin Jan et al. (2014) Figure S2a

21. Calvin Jan et al. (2014) Figure 1e

22. Calvin Jan et al. (2014) Figure S2b

23. Calvin Jan et al. (2014) Figure 1d

24. Calvin Jan et al. (2014) Figure S2c

25. Calvin Jan et al. (2014) Figure 1e

26. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 1. Validation of proximity-specific ribosome profiling. Science 7 November 2014 vol. 346 p716 1257521-1

27. 1. Validation of proximity-specific ribosome profiling. Science 7 November 2014 vol. 346 p716 1257521-1

28. Calvin Jan et al. (2014) Figure 2b

29. 1. Validation of proximity-specific ribosome profiling. Science 7 November 2014 vol. 346 p716 1257521-1

30. Calvin Jan et al. (2014) Figure 2a

31. Calvin Jan et al. (2014) Figure 2c,d

32. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 3. Co-translational targeting of SRP-dependent and -independent substrates in vivo. Science 7 November 2014 vol. 346 p716 1257521-1

33. Calvin Jan et al. (2014) Figure 3a

34. Ast Tslil, Cohen Galit, Schuldiner Maya (2013) Cell 152 p1134-1145.

35. Calvin Jan et al. (2014) Figure 3b

36. Calvin Jan et al. (2014) Supplemental Figure 7

37. Calvin Jan et al. (2014) Supplemental Figure S8.

38. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 4. Comprehensive analysis of Co- versus Post-translational translocation in vivo. Science 7 November 2014 vol. 346 p716 1257521-1

39. 4. Comprehensive analysis of Co- versus Post-translational translocation in vivo. Science 7 November 2014 vol. 346 p716 1257521-1

40. Calvin Jan et al. (2014) Figure 3c

41. Calvin Jan et al. (2014) Figure 3d

42. Calvin Jan et al. (2014) Figure 3e

43. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 5. Timing and specificity of Co-translational targeting to the ER. Science 7 November 2014 vol. 346 p716 1257521-1

44. 5. Timing and specificity of Co-translational targeting to ER. Science 7 November 2014 vol. 346 p716 1257521-1

45. Calvin Jan et al. (2014) Figure 4a

46. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 5. Timing and specificity of Co-translational targeting to the ER. (a) Ssh1. Science 7 November 2014 vol. 346 p716 1257521-1

47. Calvin Jan et al. (2014) Figure 4b

48. Calvin Jan et al. (2014) Figure 4c

49. Calvin Jan et al. (2014) Figure 4d

50. Calvin Jan et al. (2014) Figure 4e

51. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 5. Timing and specificity of Co-translational targeting to the ER. (b) Sec63. Science 7 November 2014 vol. 346 p716 1257521-1

52. Calvin Jan et al. (2014) Figure 4f

53. Calvin Jan et al. (2014) Figure 4g

54. Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling Calvin H. Jan, Christopher C. Williams, Jonathan S. Weissman 6. Dynamics of ER-associated ribosomes. Science 7 November 2014 vol. 346 p716 1257521-1

55. 6. Dynamics of ER-associated ribosomes. Science 7 November 2014 vol. 346 p716 1257521-1

56. Calvin Jan et al. (2014) Figure 5a

57. Calvin Jan et al. (2014) Figure 5b

58. Calvin Jan et al. (2014) Figure 5c