1. NCBI: something old, something new

2. What is NCBI? Create automated systems for knowledge about molecular biology, biochemistry, and genetics. Perform research into advanced methods of analyzing and interpreting molecular biology data. Enable biotechnology researchers and medical care personnel to use the systems and methods developed. On November 4, 1988 that President Ronald Reagan signed the Health Omnibus Extension Act to create The National Center for Biotechnology Information as part of National Library of Medicine at NIH.

3. The National Center for Biotechnology Information (NCBI) Part of National Library of Medicine (NLM) at National Institutes of Health (NIH) Bethesda,MD

5. NCBI: down on the farm Genome assembly and annotation Pre-computed relationships for: –Protein clusters –“Nearby” nucleic acid –EST unigene clusters –Putative homologous genes –Conserved protein domains –SNPs –Reciprocal links

6. Web users: 2.7 million per day Data downloaded: 23 TB per day Peak web hits: 7,000 per second Web page views: 26 million per day NCBI Daily Users

7. NCBI: Integrated platform for data analysis and visualization Primary Data sets: BioProject, BioSample, Genome, Assembly, Annotation Curated data sets: Refseq genomes, Gene, Protein Clusters, Pathogenic Factors, Targeted Loci Entrez search and retrieval system Analysis Tools: Annotation pipeline, genome alignments, protein clustering, multiple alignments, (assembly, SNP) Validation Tools: assembly and annotation quality assesment Visualization Tools: genomeMap, distance tree, proteinMap, Genome Comparison

8. How to navigate through NCBI resources Text search in Entrez Search in MapViewer All databases Marker/gene name Specialized databases Accession # Use limits page Organism name Sequence search Pre-computed similarities BLAST Blink Genome BLAST CDD Splign/ProSplign TaxPlot Use Genome specific resources

9. Getting Started: NCBI Home Page http://www.ncbi.nlm.nih.gov Search All Databases in Entrez Learn more: Newsletter Education mailing lists Search resources by name

10. NCBI Resources by name

11. How to: NCBI help pages

12. The NCBI Handbook

13. Keeping up with what’s new NCBI News on Bookshelf www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=newsncbi NCBI News on Bookshelf www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=newsncbi

14. Genetic Testing Registry (GTR)

15. BLAST

16. Entrez Assembly

17. Entrez Genome redesign

18. Microbial Genome Resources

19. NCBI on Facebook and Twitter

20. David Lipman on GenBank 25 th Anniversary