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Isotype ControlCD126 NA A B Supplementary Figure 1. CD126 expression on CLL cells. (A) A representative FACS plot of the isotype control and CD126 staining.

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Presentation on theme: "Isotype ControlCD126 NA A B Supplementary Figure 1. CD126 expression on CLL cells. (A) A representative FACS plot of the isotype control and CD126 staining."— Presentation transcript:

1 Isotype ControlCD126 NA A B Supplementary Figure 1. CD126 expression on CLL cells. (A) A representative FACS plot of the isotype control and CD126 staining of CLL cells immediately after ficoll. Cells were incubated with anti-CD126 and anti-CD19 antibodies for 1 hour and CD19+/CD126+ cells were detected by FACS; (B) Comparison of CD126 expression following isolation (in vivo) and after 48 hours of culture (in vitro) (n=6)

2 A C Supplementary Figure 2. In vitro and in vivo chemoresponsiveness in CLL patients with 11q-/17p- and with higher or lower CD126 expression. (A) Chlorambucil- induced cell death in 11q/17p deleted (11q-/17p-) or non-deleted CLL cells; (B) Chlorambucil-induced cell death in 11q-/17p- CLL cells with higher or lower CD126 expression; (C) Frequency distribution of the in vivo chemoresponsiveness of CLL patients with 11q-/17p- or non-deleted; (D) Frequency distribution of the in vivo chemoresponsiveness of 11q-/17p- CLL patients with higher or lower CD126 expression. CR=complete remission; PR=partial remission; SD=stable disease. CRPRSDProgress Higher CD1261513 Lower CD1263110 CRPRSDProgress 11q-17p-4623 Non deleted91211  B D

3 Supplementary Figure 3. Correlation between CD126 expression and the sensitivity to Fludarabine-induced cell death. Fresh CLL cells were incubated with 20 µg/ml of Fludarabine for 24 hours. The correlation between Fludarabine-induced cell death and CD126 expression (n=11, P<0.01) was analyzed by Pearson’s correlation. R=-0.746 p < 0.01

4 Supplementary Figure 4. Autocrine of IL-6 by fresh CLL cells. Fresh CLL cells were cultured up to 48 hours. IL-6 production was measured by ELISA (n=8).

5 p=0.97 Supplementary Figure 5. Cytotoxicity of Tocilizumab on CLL cells. Fresh CLL cells were cultured with or without Tocilizumab for 24 hours, cell viability was analyzed as PI-/Annexin V- cells (N=12).

6 Supplementary Figure 6. Chlorambucil-induced CLL cell death. Fresh CLL cells were treated with Chlorambucil at the indicated doses with or without 10 µg / ml Tocilizumab for 24 hours. Cell death was analyzed as PI+/Annexin V+ cells (N=6). (A) The cell death in two groups. (B) compare the increased cell death by Tocilizumab with different doses of CBL. AB

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