1. Cell Transport Moving things into and out of the cell through the cell membrane to maintain balance ( homeostasis ) Passive: –Doesn’t take any energy from the cell –Just happens (kinetic energy of molecules) Active: –Uses the cell’s energy, from mitochondria –ATP (Adenosine TriPhosphate)

2. Cell Memebrane Phospholipid Bilayer –2 layers, back to back, of molecules of fat called phospholipids Fluid Mosaic –Other molecules, too, like proteins, but they don’t just sit there, they move around Selectively permeable –Not just anything can pass through

3. Cell membrane

4. Diffusion Molecules simply move from an area of higher concentration to lower (with concentration gradient ) Because of the molecules’ kinetic energy (passive) Will stop when reach equilibrium (equal concentration) Molecules still move, but for each one that moves in to the cell, one moves out (no net movement)

5. Diffusion across cell membrane Happens only for very small particles (salt, etc.) Move between phospholipid molecules of the bilayer Or for molecules that are nonpolar (hydrophobic) and dissolve into the space between the two layers of the cell membrane, but not in water

6. Facilitated diffusion For molecules that are too big to get in (e.g. sugar), or have charges (ions) Proteins in the cell membrane will chemically bind to the molecule. ( specific …only 1 molecule per protein…like a lock and key) The protein will then change shape and the molecule will move in or out of the cell. Takes no energy (passive)

7. Facilitated diffusion Note: each protein is specific for one molecule, but there are many different proteins in each cell membrane

8. Osmosis Special kind of diffusion (passive) Only for water across the membrane Water can move against its concentration gradient, that is water can move from low water to high water concentration Water moves to “water down” whatever is most concentrated.

9. Osmosis If the cell has more “stuff” dissolved outside than inside, it is in a hypertonic environment It will try to “water down” the environment to make it less harsh Water leaves the cell, the cell shrinks ( plasmolysis ) http://www.youtube.com/watch? v=gWkcFU-hHUk&feature=relatedhttp://www.youtube.com/watch? v=gWkcFU-hHUk&feature=related

10. Osmosis If the cell has more “stuff” dissolved inside than outside, it is in a hypotonic environment It will try to “water down” the inside to make it less harsh Water enters the cell, the cell grows If it gets too big, it explodes ( cytolysis ) http://vimeo.com/2726364

11. Osmosis If the cell has equal amounts of “stuff” dissolved inside and out, it is in an isotonic environment Water molecules will move both in and out of the cell equally, so there is no net movement of water Notes: water still moves in and out in hypertonic and hypotonic solutions, but the net movement is in one direction

12. Equilibrium Passive transport will continue until equal concentrations are reached in and out of the cell ( equilibrium ). Molecules still move back and forth across the membrane, but do so at equal rates No net (total) movement of molecules, though

13. Active Transport Sometimes cell wants higher or lower concentrations (not equilibrium) Have to use the cell’s energy (ATP adenosine triphosphate) Moves against concentration, i.e. from low to high concentration Moves large molecules or large amounts of molecules

14. Protein Pumps Energy changes shape of the protein to move things in/out of the cell Is specific …each protein can only chemically bind with one molecule, but there are many different proteins in the cell membrane (like a lock and key) Na + K + pump

15. Protein pumps 3 sodiums are taken out of the cell and 2 potassiums brought in using ATP.

16. Transport by vesicle Moves large molecules or large quantities of something into and out of the cell Into the cell is called endocytosis – Pinocytosis : water/liquid moves – Phagocytosis : solids move Out of the cell is called exocytosis (e.g. products from Golgi Apparatus)

17. Endocytosis Note part of cell membrane makes the vesicle and is now inside the cell! http://www.youtube.com/watch?v=W6rnhiMxt KUhttp://www.youtube.com/watch?v=W6rnhiMxt KU

18. Exocytosis Note the vesicle fuses with the cell membrane and is now part of it! http://www.youtube.com/watch?v=U9pvm_4- bHghttp://www.youtube.com/watch?v=U9pvm_4- bHg

20. ATP Adenosine Triphosphate ATP Adenosine Diphosphate ADP + P + energy