1. Unit 5 Genetic Engineering

2. Unit 5.1 Moving Genes

3. Unit 5.1 Moving Genes “ the transfer of a gene from the DNA of one organism into another organism, in order to produce an organism with desired traits” a) Genetic engineering b) genetically modified organism (GMO) c) plasmid d) recombinant DNA

4. Unit 5.1 Moving Genes “ the transfer of a gene from the DNA of one organism into another organism, in order to produce an organism with desired traits” a) Genetic engineering b) genetically modified organism (GMO) c) plasmid d) recombinant DNA

5. Unit 5.1 Moving Genes “ ____that cuts DNA at a specific sequence of nucleotides” a) recombinant DNA b) gene c) restriction enzyme d) vector

6. Unit 5.1 Moving Genes “ ____that cuts DNA at a specific sequence of nucleotides” a) recombinant DNA b) gene c) restriction enzyme d) vector

7. Unit 5.1 Moving Genes “ new ___________ created by altering the genetic material (DNA) of existing organisms; usually in an attempt to remove undesirable or create desirable characteristics in the new organism” a) Genetic engineering b) genetically modified organism (GMO) c) restriction enzyme d) vector

8. Unit 5.1 Moving Genes “ new ___________ created by altering the genetic material (DNA) of existing organisms; usually in an attempt to remove undesirable or create desirable characteristics in the new organism” a) Genetic engineering b) genetically modified organism (GMO) c) restriction enzyme d) vector

9. Unit 5.1 Moving Genes “ Circular DNA molecule found in bacteria which can be inserted with foreign DNA. Used to mass produce insulin and human growth hormone” a) genetically modified organism (GMO) b) plasmid c) recombinant DNA d) vector

10. Unit 5.1 Moving Genes “ Circular DNA molecule found in bacteria which can be inserted with foreign DNA. Used to mass produce insulin and human growth hormone” a) genetically modified organism (GMO) b) plasmid c) recombinant DNA d) vector

11. Unit 5.1 Moving Genes 0 Understand key vocabulary terms 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

12. Unit 5.1 Moving Genes 0 Identify two pros and two cons for the following genetic engineering scenario: see Frankenfish Pros (2)Cons (2)

13. Unit 5.1 Moving Genes 0 Identify pros and cons to a given Genetically Modified Organism (GMO). 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

14. Unit 5.1 Moving Genes 0 Identify the restriction enzyme(s) that create(s) a “blunt-end” cut. See chart at right.

15. Unit 5.1 Moving Genes 0 Identify the restriction enzyme(s) that create(s) a “blunt-end” cut. See chart at right. 0 Hpa I

16. Unit 5.1 Moving Genes 0 Identify the restriction enzyme(s) that create(s) a “Sticky-end” cut in the DNA segment below. AATAGGATCCGC TTATCCTAGGCG

17. Unit 5.1 Moving Genes 0 Identify the restriction enzyme(s) that create(s) a “Sticky-end” cut in the DNA segment below. AATAGGATCCGC TTATCCTAGGCG

18. Unit 5.1 Moving Genes 0 Match a certain restriction enzyme (e.g. Bam HI) to its correct DNA “cut” site. (see Figure 9.8 in etext). 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

19. Unit 5.1 Moving Genes 0 Describe (and draw) a “blunt” cut made by restriction enzymes. 0 Describe (and draw) a “sticky-end” cut made by restriction enzymes.

20. Unit 5.1 Moving Genes 0 Compare and contrast “sticky” and “blunt” ends (see Figure 9.8 in etext). 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

21. Unit 5.1 Moving Genes 0 Arrange the terms DNA, protein, and gene into the three boxes below based on how they relate to each other.

22. Unit 5.1 Moving Genes 0 Arrange the terms DNA, protein, and gene into the three boxes below based on how they relate to each other. Protein DNA Gene

23. Unit 5.1 Moving Genes 0 Understand that a gene is a segment of DNA that codes for a certain protein (amino acid chain). 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

24. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

25. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism (D) 0 restriction enzyme 0 Insulin gene D B C A

26. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

27. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism (A) 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

28. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

29. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene (B) 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene (B) D B C A

30. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 vector 0 plasmid 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

31. Unit 5.1 Moving Genes 0 Describe the process of genetic engineering (or recombinant DNA technology). Include the terms: 0 desired gene 0 Vector © 0 Plasmid © 0 chromosome 0 donor organism 0 recipient organism 0 restriction enzyme 0 Insulin gene D B C A

32. Unit 5.1 Moving Genes 0 Understand that through genetic engineering, foreign DNA is introduced into a (host) organism. This foreign gene is then transcribed and translated as part of the host’s genome to produce the foreign protein. 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

33. Unit 5.2 Tools of Biotechnology

34. Unit 5.2 Biotech Tools “an organism that contains genes from other organisms is a ____________________” a) (desired) gene b) bacteria c) transgenic organism d) vector

35. Unit 5.2 Biotech Tools “an organism that contains genes from other organisms transgenic organism” a) (desired) gene b) bacteria c) transgenic organism d) vector

36. Unit 5.2 Biotech Tools “extremely small, single-celled organisms without a nucleus; prokaryotic cells” a) bacteria b) donor organism c) chromosome d) Vector

37. Unit 5.2 Biotech Tools “extremely small, single-celled organisms without a nucleus; prokaryotic cells” a) bacteria b) donor organism c) chromosome d) Vector

38. Unit 5.2 Biotech Tools “use of bacteria to clean up toxic materials” a) bacteria b) recipient organism c) vector d) bioremediation

39. Unit 5.2 Biotech Tools “use of bacteria to clean up toxic materials” a) bacteria b) recipient organism c) vector d) bioremediation

40. Unit 5.2 Biotech Tools 0 Understand key vocabulary terms 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

41. Unit 5.2 Biotech Tools 0 Describe how vectors and DNA fragments combine to make a changed (transgenic organism); refer to Figure 9.10 on page 465. 0 Especially how vector gets into recipient cell. 0 5 methods (How Can this Be Done?)

42. Unit 5.2 Biotech Tools 0 Explain how vectors and DNA fragments combine to make a changed (transgenic organism); refer to Figure 9.10 in etext 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

43. Unit 5.2 Biotech Tools 0 List the five (5) genetic engineering procedures 0 see How Can this Be Done?

44. Unit 5.2 Biotech Tools 0 List the five (5) genetic engineering procedures: 0 Gene therapy 0 Drug Therapy 0 Tissue engineering 0 Gene bullets 0 Electroporation

45. Unit 5.2 Biotech Tools 0 Identify the steps that are common to the following biotechnology methods: 0 Gene therapy 0 Drug Therapy 0 Tissue engineering 0 Gene bullets 0 Electroporation 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

46. Unit 5.2 Biotech Tools 0 Know that, in each of the five genetic engineering procedures (listed in #2), the common steps include:

47. Unit 5.2 Biotech Tools 0 Know that, in each of the five genetic engineering procedures (listed in #2), the common steps include: 0 identifying a gene of interest in the provider organism 0 using a restriction enzyme to cut out desired gene 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

48. Unit 5.2 Biotech Tools 0 Identify what is contrasting about each biotechnological method of genetic engineering: 0 Gene therapy- involves use of vector. Used for expressing a trait that was not already expressed by the organism 0 Drug Therapy – bacterial transformation and reproduction. Used to produce a drug in quantity for medicinal purposes 0 Tissue engineering – genes fused to gel-like substance. Used to heal injuries. 0 Gene bullets – involves use of vector penetrating plant cells. Used to introduce new genes into crops. 0 Electroporation – involves use of vector placed in an electrical field. Used to modify the DNA of animals or produce new crops.

49. Unit 5.2 Biotech Tools 0 Describe the process of “bioremediation”. (see p. 471)

50. Unit 5.2 Biotech Tools 0 Describe the process of “bioremediation”. (see p. 471) 0 The use of microorganisms to degrade (toxic) waste

51. Unit 5.2 Biotech Tools 0 How might it be possible to create a pair of more environmentally friendly jeans using genetically modified bacteria? (see p. 472)

52. Unit 5.2 Biotech Tools 0 How might it be possible to create a pair of more environmentally friendly jeans using genetically modified bacteria? (see p. 472) 0 Add gene for toluene dioxygenase enzyme to bacteria. Then bacteria produces blue colored indigo pigment.

53. Unit 5.2 Biotech Tools 0 Explain how vectors and DNA fragments combine to make a changed (transgenic organism) 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

54. Unit 5.2 Biotech Tools 0 Explain how vectors and DNA fragments combine to make a changed (transgenic organism); refer to Figure 9.10 in etext 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

55. Unit 5.3 Biotechnology & Society

56. Unit 5.3 Biotech & Society 0 List, sequence (put in order), and describe each of the four BDMP steps.

57. Unit 5.3 Biotech & Society 0 List, sequence (put in order), and describe each of the four BDMP steps. 0 Describe CONTEXT 0 Identify STAKEHOLDERS 0 Identify ALTERNATIVES 0 Make DECISION

58. Unit 5.3 Biotech & Society 0 List, sequence (put in order), and describe each of the four BDMP steps. 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this

59. Unit 5.3 Biotech & Society 0 Apply any, or all, of the BDMP steps to an ethical scenario related to genetic engineering. 0 A: I am ABLE to do this 0 B: I am BEGINNING to get this 0 C: I still CAN’T do this