1. Development of a Murine Perfusion System Nicola Asgill Fredrick Hilliard George Kittos

2. Objectives Langendorff perfusion system  Requirements: Miniature scale Fluid temperature controlled Inter-changeable components Drug introduction into perfusate

3. Background Langendorff Perfusion System  Simulates natural blood flow  Retrograde fluid flow Attached to aorta  Examine effects of drug on heart

4. Considerations for project Why will this work?  Controlled temperature, flow rate and pressure Improvement of heat stability Multi-drug delivery capability  Disposable components Why is this important?  Possible testing applications: Drug delivery Cardiac cell response

5. Prototype

6. Prototype Modifications for Preliminary Testing Flow will be gravity controlled  Removal of Peristaltic Pump Removal of Flow Sensor Pump and Sensor will be added based on results of preliminary testing

7. Peltier Element Max Current7.6A Max Power76 W Max Voltage16.3 V dT Max72°C Dimensions48x48x4.8mm 3

8. Peltier Element Test Goals  Show functionality – Heating capabilities  Calculate Time Constant To heat from room temperature to 37 ± 1°C  Determine appropriate input voltage

9. Peltier Element Test Conclusion:  Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp.  Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail.  Input Voltage of 12V – faster heating and the device did not malfunction

10. LabVIEW Temperature Control

11. Transparent Glass Chamber Plexiglass Visualize electric activity in heart using dyes Staggered holes

12. Transparent Glass Chamber

13. Current Work Heating Perfusate to 37 ± 1°C Manufacturing Plexiglass chamber Verify and Validate the LabView Module for controlling and maintaining constant temperature Tygon Tubing Peltier Element

14. Future Work Acquiring additional components Assembling Prototype Testing the perfusate heating apparatus Design and Test Control Valve System Harvesting the Mouse Heart

15. Thanks Questions?