1. 傅曉婷 Fu, Hsiao-Ting Molecular Pathology Lab Department of Pathology and Clinical Laboratory Kaohsiung Veterans General Hospital

3. Identify HazardsEvaluate RisksDetermine Necessary ControlImplement Control MeasuresEvaluate Effectiveness of Controls Biological Agents Risk Group Biological Safety Level of the Lab Containment Plans

13. http://oregonstate.edu/ehs/lvsg-bsc-type

16. Spill Out Clean-Up Procedures – Inside the BSC – Outside the BSC – Inside the Lab Equipments Transport of Infectious Substances

17. 12 34

18. 5 7 6 8

19. 9 11 10

21. Chlorine-releasing compounds Chlorine (NaOCl) Sodium dichloroisocyanurate Chloramines Chlorine dioxide Bleach, 1 g/L or 5g/L. FormaldehydeKills all microorganisms and spores, not Prions. GlutaraldehydeFaster acting than formaldehyde, but it takes several hours to kill bacterial spores. Phenolic compoundsActivity against mycobacteria, but not active against spores Quaternary ammonium compoundsSome vegetative bacteria and lipid-containing viruses AlcoholsActive against bacteria, fungi and lipid-containing viruses but not against spores. 70% for highest effectiveness. Iodine and iodophorsSimilar to that of chlorine. Hydrogen peroxideSafer than chlorine, ready-to-use 3% solution.