2. DNA Replication – Exciting stuff from chapter 10!

3. Ch 10 DNA Replication, Baby! DNA workshop – interactive basic overview: http://www.pbs.org/wgbh/aso/tryit/dna/shockwave.html Link to replication animation w/ no sound: http://www.johnkyrk.com/DNAreplication.html Link to genetically engineered corn video: http://www.youtube.com/watch?v=n9xpKx03g9M&feature=player_embedded Link to narrated DNA replication http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapter14/animations.html#http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapter14/animations.html# Link to DNA replication http://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht mhttp://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht m

4. Review Structure The "rungs" are the nitrogenous base pairs a. complementary base pairing Uniform diameter

5. Review DNA function Stores information Mutates

6. Replicates Expressed

7. DNA REPLICATION When and where? How about in bacteria?

8. Bacteria replication of DNA: one origin of replication

9. DNA replication in eukaryotes: Contain hundreds of origins and complexes make replication “________” that eventually fuse

10. not Replication is called______ _________, because one half of the original strand is always saved, or "conserved"

12. Process of DNA replication: 1. enz. helicase unwinds and breaks H-bonds 2. single-stranded binding proteins stabilize the molecule

13. 3. enz. _________ adds a small piece of _____ (called a primer) to origin of replication (DNA nucleotides can only be added to the end of an _________ chain)

14. 4. enz. DNA ____________adds new dNTPs to the ____ end - A-T, G-C, 5'  3 direction of growing strand

15. leading strand - one primer needed lagging strand - Okazaki fragments - primer needed for each fragment

18. 5. A dif. DNA polymerase replaces RNA ___________ w/ DNA 6. enz. DNA ________ joins Okasaki fragments together

21. Ch 10 DNA Replication, Baby! DNA workshop – interactive basic overview: http://www.pbs.org/wgbh/aso/tryit/dna/shockwave.html Link to replication animation w/ no sound: http://www.johnkyrk.com/DNAreplication.html Link to genetically engineered corn video: http://www.youtube.com/watch?v=n9xpKx03g9M&feature=player_embedded Link to narrated DNA replication http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapter14/animations.html#http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapter14/animations.html# Link to DNA replication http://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht mhttp://www.wiley.com/college/boyer/0470003790/animations/replication/replication.ht m

22. Telomeres - repetitive sequences of nucleotides at the ends of DNA shorten w/ each rep. purpose?

23. Enz. ______________extends telomeres in certain cells 1. stem cells 2. cancer cells

24. Excision repair - repair of damage due to chemicals, radiation, X-rays, UV light What is the result of uncorrected errors? Can mistakes that occur to DNA be repaired?