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Phenotype Testing and SDS- PAGE Mod2 Day4 Thursday Oct 16th, 2008.

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Presentation on theme: "Phenotype Testing and SDS- PAGE Mod2 Day4 Thursday Oct 16th, 2008."— Presentation transcript:

1 Phenotype Testing and SDS- PAGE Mod2 Day4 Thursday Oct 16th, 2008

2 First let’s look at colony PCR results

3 SDS-PAGE You will make a whole cell extract from equal # of cells --> OD600 Glass beads/SDS/BME/boiling steps Gel separates proteins based on size May want to change lane order for + control After 1 hour run, blot to nitrocellulose and block with milk solution until next week.

4 Yeast Phenotype Testing Choose plates you will use and put them in the incubator to dry out before running your protein gel Spot test demo…

5 For next time… What size is the protein you’re looking for (TAP = +20KDa) Make a figure of colony PCR result Calculate number of cells for RNA extraction

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