1. Diane Mary Grace T. Baguio Dafny Claire E. Quilo

2. The Study Hypoglycemic effect of the plant, Origanum vulgare through infusing different concentrations from its extracts.

3. Statement of the Problem 1. What is the difference between the baseline blood sugar and the blood sugar of the test subject after ssome time approval (after 1 hour and after 2 hours) utilizing the following: a. 100% Pure Oregano Extract b. 75% Oregano Extract solution c. 50% Oregano Extract solution d. 25% Oregano Extract solution e. insulin f. tap water only

4. Statement of the Problem 2. Is there a significant difference on the hypoglycemic effect of insulin to each of the different extract solution on: a. The first hour of introduction b. The second hour of introduction 3. Is there a significant difference on the hypoglycemic effect of tap water to each of the different extract solution on: a. The first hour of introduction b. The second hour of introduction

5. Statement of the Problem 4. Is there a significant difference on the hypoglycemic effect of the different oregano extract concentrations on the test subject: a. The first hour of introduction b. The second hour of introduction 5. Which of the extract solutions is most/least effective in terms of their hypoglycemic effect towards the subject: a. After one hour upon administration of extract b. After two hours upon administration of extract

6. Scope and Delimitation The research will cover only the hypoglycemic effect of the Origanum vulgare. The said plant will be taken from the vicinity of Iligan City. The researchers shall only use extracts obtained from the leaves of Origanum vulgare. Likewise, this study is limited only to 25%, 50%, 75%, and 100% concentrations of the extract which are tested to white mice.

7. Scope and Delimitation The negative control of this study would be the tap water while the positive control that will be used is the insulin. Introduction of the test samples will be done using a syringe.

8. Procedure CRITERIA FOR SELECTING SPECIMEN EXTRACTION PROCESS TREATMENT PROCEDURE APPLICATION OF POSITIVE AND NEGATIVE CONTROL STATISTICAL TOOLS FOR DATA ANALYSIS

9. Results After 1 hourAfter 2 hours Insulin 95.4107 Tap Water -78-32.2 100% Pure Extract 29.637.6 75% Extract Solution 21.249.6 50% Extract Solution 55.261 25% Extract Solution 46.454.6

10. SourceSSDfMSFP-value Treatmen t 14,533.8443,633.46023.382.66x10 -7 Error3,108.4020155.420 Total17,642.2424 Insulin25% extract50% extract100% extract75% extract Mean21.631.837.845.291.2 t-valuep-valuet-valuep-valuet-valuep-valuet-valuep-valuet-valuep-value Insulin 21.6 25% extract 31.81.29.2105 50% extract 37.82.05.05320.76.4556 100% extract 45.22.99.00721.70.10470.94.3592 75% extract 91.28.832.47x10 -8 7.532.91 x10 -7 6.771.38 x10 -6 5.831.04 x10 -5

11. SourceSSDfMSFP-value Treatmen t 41025.36410256.3425.911.15x10 -7 Error7916.0020395.80 Total48941.3624 25% extract50% etract100% extract75% extractTap Water Mean31.837.845.291.2138.2 t-valuep-valuet-valuep-valuet-valuep-valuet-value p- value t-value p- value 25% extract 31.8 50% extract 37.80.48.6386 100% extract 45.21.06.2996 0.59.5630 75% extract 91.24.72.0001 4.24.0004 3.66.0016 Tap Water 138.2 8.464.9x10 -8 7.981.2x10 -7 7.393.87x10 -7 3.74.0013

12. SourceSSDfMSFP-value Treatmen t 7,456.9641,864.2408.630.0003 Error4,318.0020215.900 Total11,774.9624 Insulin25% extract50% etract 100% extract75% extract Mean10.031.632.037.264.0 t- valuep-value t- value p- value t- value p- value t- value p- value t- value p- value Insulin10.0 25% extract31.62.32.0308 50% extract32.02.37.02810.04.9661 100% extract37.22.93.00830.60.55350.56.5820 75% extract64.05.81 1.10x10 -5 3.49.00233.44.00262.88.0092

13. SourceSSDfMSFP-value Treatmen t 14,048.9643,512.2408.930.0003 Error7,863.220393.16 Total21,912.1624 25% extract50% etract100% extract75% extractTap Water Mean31.632.037.264.092.4 t- value p- value t- value p- value t- value p- value t- value p- value t- value p-value 25% extract 31.6 50% extract 32.00.03.9749 100% extract 37.20.45.6600 0.41.6828 75% extract 64.02.58.0177 2.55.0190 2.41.0451 Tap Water92.44.85.00014.82.00014.40.00032.26.0348

14. SourceSSDfMSFP-value Treatmen t 10,957.833,652.620.121.12x10 -5 Error2,905.216181.575 Total13,86319 25% extract50% etract100% extract75% extract Mean31.837.845.291.2 t- value p- value t- value p- value t- value p- value t- value p- value 25% extract31.8 50% extract37.80.70.4915 100% extract45.21.57.13540.87.3981 75% extract91.26.97 3.16x10 -6 6.27 1.13x10 -5 5.40.0001

15. SourceSSDfMSFP-value Treatmen t 3,563.231,187.74.400.0194 Error4,31816269.875 Total7,881.219 25% extract50% etract100% extract75% extract Mean31.632.037.264.0 t- value p- value t- value p- value t- value p- value t- value p- value 25% extract 31.6 50% extract 32.00.04.9698 100% extract 37.20.54.5973 0.50.6235 75% extract64.03.12.00663.08.00722.58.0202

16. Conclusion Results show that oregano extract solutions have hypoglycemic effect despite the extract concentration. It tells that 25% extract solution is most effective and 75% extract solution is least effective for both time intervals.

17. Recommendations The researchers would like to recommend further studies which include the following: a) study other plants that could be possible in lowering blood sugar levels b) initiate future researches about the medicinal purposes and the chemical profile of oregano or Origanum vulgare c) look into the bioactive compounds of oregano that could have caused the hypoglycaemic effect.