1. Chloroplast Evidences 1, 2, 3

2. Evidence 1 Do not move to evidence 2 until instructed to do so

3. Evidence 1 Watch the video before viewing the plant under the microscope: https://www.youtube.com/watch?v=BB5rvjZzgFU https://www.youtube.com/watch?v=XTQhaCvlWeo

4. Ask your partner Before moving to the next slide, ask your partner: What color are chloroplasts? Move to the next slide when you and your partner agree on an answer

5. Ask your partner Before moving to the next slide, ask you partner: What shape are chloroplasts? Move to the next slide when you and your partner agree on an answer

6. Ask your partner Before moving to the next slide, ask you partner: How might structure be related to function (how does its shape relate to what it does for a cell?) Move to the next slide when you and your partner agree on an answer

7. Evidence 2 Do not move to evidence 3 until instructed to do so

8. Evidence 2 A scientist named T. G. Engelmann wanted to see if oxygen was produced in a cell and where. He knew of a bacteria called Pseudomonas is very active in the presence of oxygen and moves toward oxygen when it is present.

9. Evidence 2: Method 1)He placed plant cells from a plant called Spirogyra on a microscope slide. This plant has a spiral shaped chloroplast.

10. Evidence 2: Method 2) He put Pseudamonas bacteria on the slide with the Spirogyra plant cells and exposed it to light.

11. Ask your partner Before moving to the next slide, ask you partner: What are Pseudomonas bacteria attracted to? Move to the next slide when you and your partner agree on an answer

12. Evidence 2: Results Findings: When Engelmann shined light on the plant cell, he found that the amount of active bacteria near the chloroplast increased. The bacteria, which are attracted to oxygen, grouped around the edge of the cell where the chloroplast was located and were very active.

13. Ask your partner Before moving to the next slide, ask you partner: Where did the Pseudamonas bacteria move to? Move to the next slide when you and your partner agree on an answer

14. Ask your partner Before moving to the next slide, ask you partner: Why did it move there? Move to the next slide when you and your partner agree on an answer

15. Ask your partner Before moving to the next slide, ask you partner: How is Oxygen related to the experiment? Did the amount of Oxygen increase, decrease, or stay the same throughout the experiment? How do you know? Move to the next slide when you and your partner agree on an answer

16. Ask your partner Before moving to the next slide, ask you partner: What was the independent variable in the experiment (what did Engelmann change?) Move to the next slide when you and your partner agree on an answer

17. Ask your partner Before moving to the next slide, ask you partner: What happens when light is shined on a chloroplast? Move to the next slide when you and your partner agree on an answer

18. End of evidence 2 Do not move to evidence 3 until instructed to do so

19. Do not move to evidence 4 until instructed to do so Evidence 3

20. Chloroplast Evidence 3

21. 1) We want to find out if the cells in different kinds of tissues have chloroplasts in them 2) We want to test many different kinds of organisms. 3) If they have chloroplasts we want to know if there are a large or small amount of chloroplasts in the cells. Experimental Goals

22. Organisms Evaluated Oak Tree Dog Starfish Moss Tulip Human Algae Cactus Snail

23. Ask your partner Before moving to the next slide, ask you partner: Why would it be important to choose plants and animals in this study? Move to the next slide when you and your partner agree on an answer

24. To test the tissues we are going to take a sample and look at it under the microscope. If there are chloroplasts we will be able to see them. As you click through the next slides, record whether the samples have Many chloroplasts, few chloroplasts, or no chloroplasts Procedure

25. Oak Tree

26. Oak Tree Leaves You Found MANY Chloroplasts!!

27. Oak Tree Trunk You Found FEW Chloroplasts.

28. Dog

29. Dog Skin Cells No Chloroplasts.

30. Dog Nose Cells No Chloroplasts.

31. Dog Stomach Cells No Chloroplasts.

32. Dog Ear Cells No Chloroplasts.

33. Ask your partner Before moving to the next slide, ask you partner: Why do you think we tested more than one spot on the dog’s body? Move to the next slide when you and your partner agree on an answer

34. Moss

35. Moss Cells You Found MANY Chloroplasts!!

36. Now that we have seen the number of chloroplasts of a few organisms, make a prediction with your partner if the organism will have:  Many chloroplasts  Few chloroplasts  No chloroplasts before viewing the microscope evidence. **Remember to give reasoning when making a prediction PREDICTION Procedure

37. Tulip

38. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

39. You Found MANY Chloroplasts!! Tulip Leaf Cells

40. You Found FEW Chloroplasts!! Tulip Flower Cells

41. Ask your partner Before moving to the next slide, ask you partner: How are a tulip leaf and a tulip flower different? Why do you think there were many chloroplasts in the leaf cells and few in the flower cells? Move to the next slide when you and your partner agree on an answer

42. Human

43. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

44. Human Skin Cells No Chloroplasts.

45. Human Muscle Cells No Chloroplasts.

46. Snail

47. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

48. Snail Skin Cells No Chloroplasts.

49. Starfish

50. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

51. Starfish Cells No Chloroplasts.

52. Cactus

53. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

54. Cactus Cells You Found MANY Chloroplasts!!

55. Cactus Root Cells You Found FEW Chloroplasts.

56. Ask your partner Before moving to the next slide, ask you partner: How are cactus cells and cactus root cells different? Why do you think there were many chloroplasts in the cactus cells and few in the root cells? Move to the next slide when you and your partner agree on an answer

57. Algae

58. Ask your partner: Predictions Before moving to the next slide, make a prediction with your partner: Will this organism have many chloroplasts, few chloroplasts, or no chloroplasts? WHY?? Move to the next slide when you and your partner have provided solid reasoning for your prediction

59. Algae Cells You Found MANY Chloroplasts!!

60. CELLSFINDINGS Oak tree leaves Many chloroplasts Oak tree trunk Few chloroplasts Dog skin No chloroplasts Dog nose No chloroplasts Dog stomach No chloroplasts Dog ears No chloroplasts Moss Many chloroplasts Tulip leaf Many chloroplasts Tulip flower Many chloroplasts Human skin No chloroplasts Human muscle No chloroplasts Snail skin No chloroplasts Starfish No chloroplasts Cactus Many chloroplasts Cactus root Few chloroplasts Algae Many chloroplasts Results

61. Did you collect data for both plants and animals?

62. Ask your partner Before moving to the next slide, ask you partner: What organisms had the most chloroplasts? Move to the next slide when you and your partner agree on an answer

63. Ask your partner Before moving to the next slide, ask you partner: What organisms had the fewest chloroplsts? Move to the next slide when you and your partner agree on an answer

64. Ask your partner Before moving to the next slide, ask you partner: What is similar amongst the organisms that have a lot of chloroplasts? List as many reasons as you can. Move to the next slide when you and your partner agree on an answer

65. Ask your partner Before moving to the next slide, ask you partner: What is similar amongst the organisms that have no chloroplasts? List as many reasons as you can. Move to the next slide when you and your partner agree on an answer

66. Ask your partner Before moving to the next slide, ask you partner: Why do you think some organisms have chloroplasts and others don’t? Provide reasoning for your response. Move to the next slide when you and your partner agree on an answer

67. Do not move to evidence 4 until instructed to do so End of Evidence 3

68. Do not move to evidence 5 until instructed to do so Evidence 4