1. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 1 of 6 _____________________________________________ Synthetic Biology Review A Synthetic Oscillatory Network of Transcriptional Regulators – ‘The Repressilator’ Elowitz, M.B. & Leibler, S. Nature, vol. 403, 20 th January 2000

2. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 2 of 6 _____________________________________________ The Basics

3. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 3 of 6 _____________________________________________ The Good Results...

4. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 4 of 6 _____________________________________________...and the Other Results ◄ Limitations (c): » Accumulation of GFP as a result of limited elimination » Green – oscillation with a reduced period » Blue – several periods skipped entirely ◄ Controls (f): » Cells containing only the reporter plasmid Stochastic effects…

5. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 5 of 6 _____________________________________________ ◄ iGEM Solution: » Novel approach based on Lotka-Volterra model of predator-prey interaction » Constitutively repressed ‘predator’ cell is anti-repressed by product of ‘prey’ cell » Prey cell is repressed by product of predator cell… » Thus a stable oscillation cycle is built up Conclusions & Extensions Cell A Lux R Lux I Plux A A Cell B LuxR AA aiiA Plux AiiA LuxR

6. Tom Adie, Erika Cule & Rajdeep Ghataorhe 15/II/2008 Slide 6 of 6 _____________________________________________ The Network Model Deterministic Continuous / Stochastic Discrete Continuous model: