1. Osmosis: Striking a Balance

2. Maintaining A Balance Cells are surrounded by watery solutions and are filled by watery solutions. Cells are surrounded by watery solutions and are filled by watery solutions. A solution is a mixture in which one or more substances (Solutes) are dissolved in another substance (Solvent) A solution is a mixture in which one or more substances (Solutes) are dissolved in another substance (Solvent) The concentration of a solute is important to organisms. The concentration of a solute is important to organisms. Organisms cannot live unless the concentration of dissolved substances stays within a narrow range. Organisms cannot live unless the concentration of dissolved substances stays within a narrow range.

3. Balance and Homeostasis This balance of conditions is called Homeostasis This balance of conditions is called Homeostasis The movement of water across the membrane helps to balance the concentrations of dissolved substances in a cell The movement of water across the membrane helps to balance the concentrations of dissolved substances in a cell The diffusion of water across a selectively permeable membrane is called osmosis The diffusion of water across a selectively permeable membrane is called osmosis

4. Osmosis Like other molecules the movement of water is controlled by differences in concentration (a concentration gradient) Like other molecules the movement of water is controlled by differences in concentration (a concentration gradient) Water will move in the direction where there is a high concentration of solute (hence a lower concentration of water) Water will move in the direction where there is a high concentration of solute (hence a lower concentration of water) A simple rule to remember is SALT SUCKS A simple rule to remember is SALT SUCKS Salt is a solute, when it is concentrated inside or outside a cell, it will draw water in its direction Salt is a solute, when it is concentrated inside or outside a cell, it will draw water in its direction

6. Tonicity The measurement of solute concentration on either side of a membrane is known as Tonicity The measurement of solute concentration on either side of a membrane is known as Tonicity The tonicity of a cell’s environment affects its rate of osmosis. The tonicity of a cell’s environment affects its rate of osmosis.

7. Isotonic In an isotonic solution, the concentration of solute is the same on both sides of the membrane (inside the cell and outside). In an isotonic solution, the concentration of solute is the same on both sides of the membrane (inside the cell and outside). A cell placed in an isotonic solution neither gains or loses water. A cell placed in an isotonic solution neither gains or loses water. Most cells in the body are in an isotonic solution. Most cells in the body are in an isotonic solution. Why? Why?

8. Red Blood Cells Plant Cells

9. Isotonic Solution Isotonic: The concentration of solutes in the solution is equal to the concentration of solutes inside the cell. Result: Water moves equally in both directions and the cell remains same size! (Dynamic Equilibrium) Osmosis Animations for isotonic, hypertonic, and hypotonic solutions Osmosis

10. Hypertonicity A hypertonic solution is one that has a high solute (Less water) concentration. A hypertonic solution is one that has a high solute (Less water) concentration. Cells in a hypertonic solution will lose water. Cells in a hypertonic solution will lose water.

11. Hypertonicity As a result of a difference in structure, plant and animal cells respond differently Animal Cells shrink crenate Plant cells, maintain shape because of their cell wall, the cell membrane breaks away from the cell wall resulting in Plasmolyis

12. Red Blood Cells Plant Cells

13. Hypotonicty A hypotonic solution is one that has less solute (more water). A hypotonic solution is one that has less solute (more water). When placed in a hypotonic solution, cells tend to gain water and swell When placed in a hypotonic solution, cells tend to gain water and swell

14. Hypotonicty If an animal cell swells too much it will burst. This is called Lysis. If an animal cell swells too much it will burst. This is called Lysis. Plant cells rarely burst because of their cell wall. As water fills the cell the membrane is pushed up against the wall (turgor pressure) making the plant cell turgid. Plant cells rarely burst because of their cell wall. As water fills the cell the membrane is pushed up against the wall (turgor pressure) making the plant cell turgid.

15. Red Blood Cells Plant Cells

16. Hypotonic Solution Hypotonic: The solution has a lower concentration of solutes and a higher concentration of water than inside the cell. (Low solute; High water) Result: Water moves from the solution to inside the cell): Cell Swells and bursts open (cytolysis)! Osmosis Animations for isotonic, hypertonic, and hypotonic solutions Osmosis

17. Hypotonicity Many single cell organisms live in hypotonic solution so they may be in danger of constantly bursting. Many single cell organisms live in hypotonic solution so they may be in danger of constantly bursting. To help these organisms they have a contractile vacuole to pump water out of the cell. To help these organisms they have a contractile vacuole to pump water out of the cell.

18. What type of solution are these cells in ? A CB HypertonicIsotonicHypotonic

23. Questions How can we determine which solution is isotonic to a given set of cells? How can we determine which solution is isotonic to a given set of cells? Is the concentration the same for all cells? Is the concentration the same for all cells?