1. IsolateName of each RNA genome sequenceHost plantRegionRNA genomeAccession # TSWV-1TSWV Tomato NJ-JN TSWV_1_LTomatoNa-Ju (NJ) city, Jeon-Nam (JN) provinceLHM581934 TSWV-1TSWV Tomato NJ-JN TSWv_1_MTomatoNa-Ju (NJ) city, Jeon-Nam (JN) provinceMHM581935 TSWV-1TSWV Tomato NJ-JN TSWV_1_STomatoNa-Ju (NJ) city, Jeon-Nam (JN) provinceSHM581936 TSWV-2TSWV Pepper1 CY-CN TSWV_2_LPepperChung-Yang (CY) city, Chung-Nam (CN) provinceLHM581937 TSWV-2TSWV Pepper1 CY-CN TSWV_2_MPepperChung-Yang (CY) city, Chung-Nam (CN) provinceMHM581938 TSWV-2TSWV Pepper1 CY-CN TSWV_2_SPepperChung-Yang (CY) city, Chung-Nam (CN) provinceSHM581939 TSWV-3TSWV Pepper2 CY-CN TSWV_3_LPepperChung-Yang (CY) city, Chung-Nam (CN) provinceLHM581940 TSWV-3TSWV Pepper2 CY-CN TSWV_3_MPepperChung-Yang (CY) city, Chung-Nam (CN) provinceMHM581941 TSWV-3TSWV Pepper2 CY-CN TSWV_3_SPepperChung-Yang (CY) city, Chung-Nam (CN) provinceSHM581942 Supplementary Table S1. GenBank accession numbers of individual RNA segments in TSWV-1, TSWV-2, and TSWV-3 in this study

2. Primer namePrimer sequence (5′  3′) Size of PCR product (bp) TSWV-L-1FAGAGCAATCAGGTAACAACG 1257 bp TSWV-L-1RTTTTGGATTAAGTGGTCTTTC TSWV-L-2FGAAGATATTGAAAGAATAATTGATTC 1101 bp TSWV-L-2RATTCAGGATTTTTAGCATAATTATTG TSWV-L-3FCAAGTATAGACATGTCTTCTCTGACTC 1286 bp TSWV-L-3RCCTAGATAGTAATCAGATAATGCTTTC TSWV-L-4FTGAAGACATATACCCTAAGAAAGC 976 bp TSWV-L-4RCAGATAGGAAAGCCAATCTTG TSWV-L-5FCATCGGAAGCCATATCTATAAG 1158 bp TSWV-L-5RGCTCTCTGAATCTCATCTGTAGATA TSWV-L-6FCTCCTATAGAGCCGTTGTCTATATTAG 1161 bp TSWV-L-6RATCATATAATTGCATGCTTTCACC TSWV-L-7FTTGGAACAGGTTTAATCATGG 1276 bp TSWV-L-7RTTCTCTGATATCATCATCTACAATCTC TSWV-L-8FGAATGACCACAGACAACAAAATG 1131 bp TSWV-L-8RCAATCAAGAACTTTATCAACTCACTTAG TSWV-L-9FGTGTTGAGGCTAGATGAGGAAG 916 bp TSWV-L-9RAGAGCAATCAGGTACAACTAAAAC TSWV-M-1FAGAGCAATCAGTGCATCAGA 955 bp TSWV-M-1RCTTCTTCTTCAACTGATCTCTCAAG TSWV-M-2FGCAAGCTGATAATTCCTAAAGG 1351 bp TSWV-M-2RAAGGAGATGACATGTCTTGGG TSWV-M-3FCCGCATAGAAGACAGCC 1276 bp TSWV-M-3RGTTATAGAAGGTCCTAATGATTGCA TSWV-M-4FGTTAACCCTAAAGAGCTTCCTG 979 bp TSWV-M-4RGAGAAGATCATGGGTTATTTGAT TSWV-M-5FCTTATCCAAGAAAAATTGATGC 1051 bp TSWV-M-5RAGAGCAATCAGTGCAAACAAAA TSWV-S-1FAGAGCAATTGTGTCATAATTTTATTC 1258 bp TSWV-S-1RTTGCAGATATCTTCACTGTAATTTAAG TSWV-S-2FCTCTGCTTGAAACTCACACATC 1097 bp TSWV-S-2RAAATGAATGAAGATCAGGTGAAG TSWV-S-3FTCCATAGCAATACTTCCTTTAGC 848 bp TSWV-S-3RAGAGCAATTGTGTCAATTTTATTC Supplementary Table S2. List of oligonucleotide primers used for sequencing of TSWV-1, TSWV-2, and TSWV-3

3. RNA segmentName of proteinLength of nucleotide sequencesTSWV-1TSWV-2TSWV-3 LRdRp8640 bp34-8673 MNSm762 bp248-1009 MGn-Gc3408 bp1292-46991277-4684 SNSs1404 bp89-1492 SN777 bp2041-28172086-2862 Supplementary Table S3. Nucleotide positions for individual protein of three TSWV isolates originated from Korea

4. L RNA IsolateTSWV-BR-01TSWV-1TSWV-2TSWV-3 TSWV-BR-0195.1%93.2%93.3% TSWV-195.1%93.4%93.5% TSWV-293.2%93.4%99.8% TSWV-393.3%93.5%99.8% M RNA IsolateTSWV-BR-01TSWV-1TSWV-2TSWV-3 TSWV-BR-0190.7%90.8%90.7% TSWV-190.7%96.1%96% TSWV-290.8%96.1%99.9% TSWV-390.7%96%99.9% S RNA IsolateTSWV-BR-01TSWV-1TSWV-2TSWV-3 TSWV-BR-0193%91.4% TSWV-193%95.4%95.3% TSWV-291.4%95.4%99.9% TSWV-391.4%95.3%99.9% Supplementary Table S4. Nucleotide sequence identity of three TSWV isolates in this study as compared to BR-01 isolate