1. Mrs. B’s Biology version of the Maury Povich show

2.  Paternity Test BioKit  Carolina #70-0130  Simplified paternity test using blood serums  Includes materials for 30 students  Cost $96.00

3.  Learned about A/B/O blood types  Learned about Rh factors  Completed the Nobel Prize blood typing online activity  http://nobelprize.org/educational/medicine/landst einer/ http://nobelprize.org/educational/medicine/landst einer/

4.  Purpose of this lab is to determine the blood groups of the mother (Betty), her natural daughter (Abby), and three possible fathers.  Students are in partners – one student is the “ABO investigator” and the other student is the “Rh investigator”

5. Agglutination occurs if a granular film appears on the blood  Blood reacting to anti-A is group A  Blood reacting to anti-B is group B  Blood reacting to both anti-A and anti-B is group AB  Blood not reacting to either anti-A or anti-B

6.  Blood reacting to anti-Rh(D) is Rh+  Blood not reacting to anti-Rh(D) is Rh-

7.  A lab handout  A partner  A test tray (one per partnership)  10 stirring sticks (per partnership)

9. Mother (Betty) Child (Abby) “Father #1” “Father #2” “Father #3” Anti-ANoYes NoYes Anti-BYes NoYes Anti- Rh(D) NoYesNo Yes ABO Group BABAB Rh(D) Group Rh-Rh+Rh- Rh+

10.  After ABO group tests, #1 and #3 could both possibly be the fathers. #2 is NOT the father  After the Rh(D) group tests, #1 could not be the father  #3 is very possibly the father.

11.  At this point, discuss that in an actual paternity test many other blood factors would be tested  Discuss the fact that when sufficient factors are tested, all nonfathers can be excluded

12.  PROS  Agglutination is obvious with A/B/O  Easy to reproduce  High interest  CONS  Rh agglutination takes 3-5 minutes to get good results  Kit comes with a small amount of serum...order more and reproduce on your own