1. Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes find the genes! “FIRST” generation sequencing

2. M.L. Metzker 2010. Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs. Ion torrent Nanopore

3. 5 Selected Technologies. 2 nd, 3 rd, and 4 th generation sequencing. Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, 4327. Solexa - - ->Illumina

7. Allows both directions: “pair-end”

9. Life: Roche/454; Polonator Soon to be bought by Thermo Fischer

12. NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

13. Ion Torrent (BIU) http://www.iontorrent.com/1-trillion-front-end/

18. Pacific Biosciences

21. New Generation: Template With hairpins On each end

23. Nanopore DNA sequencing

24. http://lifetech-it.hosted.jivesoftware.com/videos/1016 ??? work