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Template preparation EE Slawson Tempel, © WUSTL. Template preparation EE Slawson Tempel, © WUSTL.

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Presentation on theme: "Template preparation EE Slawson Tempel, © WUSTL. Template preparation EE Slawson Tempel, © WUSTL."— Presentation transcript:

1 Template preparation EE Slawson Tempel, © WUSTL

2 Template preparation EE Slawson Tempel, © WUSTL

3 Template preparation EE Slawson Tempel, © WUSTL

4 Template preparation EE Slawson Tempel, © WUSTL

5 Template preparation EE Slawson Tempel, © WUSTL

6 © Roche, EE Slawson Tempel, © WUSTL

7 EE Slawson Tempel, © WUSTL

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25 Fragment DNA 454 – Paired end reads EE Slawson Tempel, © WUSTL

26 Attach adapters 454 – Paired end reads EE Slawson Tempel, © WUSTL

27 Ligate in a circle 454 – Paired end reads EE Slawson Tempel, © WUSTL

28 Digest with restriction enzyme 454 – Paired end reads EE Slawson Tempel, © WUSTL

29 Isolate fragments containing adapter 454 – Paired end reads EE Slawson Tempel, © WUSTL

30 Attach sequencing adapters and sequence 454 – Paired end reads EE Slawson Tempel, © WUSTL

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