1. Laboratory Animal Handling Technique

2. Escape easily Very likely to bite

4. Full Restraint Scruffing Technique  Hold by tail base  Use forefinger and thumb to gently pin down the head  Using the same fingers, grasp a sufficient amount of loose skin over the neck  Hold the tail with the little finger of the same hand for additional support

5. Restraint http://www.bva- awf.org.uk/resources/tutorials/BVA0 5-Mouse/Mouse.html http://www.bva- awf.org.uk/resources/tutorials/BVA0 5-Mouse/Mouse.html

6. Laboratory Animal Handling Technique - Mouse A. Blood collection from tail vein B. Blood collection from orbital sinus C. Blood collection from cardiac puncture D. Blood collection from saphenous vein E. Intraperitoneal injection F.Subcutaneous injection G. Oral Feeding H. Sexing

7. Blood Collection From Tail in Mouse For collection of small amount of blood (Approximate 0.1 ml )

8. Tools for Blood Collection from Tail 75% alcohol cotton ball for surface disinfection Small plastic bottle with 1/2 cm diameter holes in both ends as mouse restrainer Scissors Pipetteman and tips A vial for blood collection

9. Placing a mouse on a cage lid and grasping the loose skin behind the ears by the thumb and forefinger

10. Push the mouse into the restrainer

11. Leave the tail of the mouse outside the cover of the restrainer

12. Amputate the tip of the mouse tail by scissors

13. Massage the tail and collect blood by pipetteman

14. Blood Collection From Retro Orbital Sinus in Mouse Should apply anesthetic before blood withdraw A convenience and easy apply method for blood collection in mouse Collect amount up to 0.5 ml

15. Tools for Blood Collection from Orbital Sinus in Mouse 75% alcohol cotton ball for surface disinfection anesthesia Glass capillary tube and vial for blood collection

16. Anesthetize a mouse by intraperitoneal injection

17. Retro-orbital Sinus Blood Collection The retro-orbital sinus is the site located behind the eye at the medial or lateral canthus. This venous sinus is located just underneath the conjunctival membrane.

18. Use a glass capillary tube to penetrate the orbital conjunctiva and rupture the orbital sinus

19. With a gentle rotating motion, insert the tube through the sinus membrane

20. Collect blood with a vial

21. Blood Collection From Cardiac Puncture in Mouse For collect up to 1 ml of blood within a short period of time Must be performed under general anesthetic or post-mortem

22. Tools for Cardiac puncture in Mouse 75% alcohol cotton ball for surface disinfection anesthesia, euthanasia protocol 25ga needle with 1-3 ml syringe for blood withdraw

23. Disinfect the thorax area with 75% alcohol cotton ball

24. Search for the maximum heart palpitation with your finger

25. Insert the needle at the base of the sternum, bevel up, into the thoracic cavity at a 15 - 20° angle directed just to the left of the midline. Aspirate slowly

26. Insert a 24G 1” needle through the thoracic wall at the point of maximum heart palpitation

27. Withdraw blood slowly by your right hand