1. Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast and endospore stains Review the structure and function of endospores

2. Modified Kinyoun’s Acid-fast Stain – Cold Method Heat fix smear Primary stain: Carbol fuchsin for AFB, (5 min) Rinse with water Decolorize briefly (~10 drops) with acid alcohol (~ 30 sec) Immediately rinse with water Counterstain: Methylene blue (1 min) Rinse with water

3. For Acid Fast Bacteria Use only Carbol fuchsin for AFB!! Acid Alcohol Methylene Blue

4. Student slide from SS 2015

8. Endospore Stain Heat fix smear Primary stain: Malachite green, steam (5 min) Rinse thoroughly with water (~ 30 sec) Counterstain: Safranin (1 min)