1. MOL The Mission Operations Laboratory MOL The Mission Operations Laboratory NASA MSFC Huntsville, Alabama Preliminary Advanced Colloids Experiment – 2 Payload Overview (PACE2) 19 July 2010 Wayne Wright EO20 256-961-1602 wayne.c.wright@nasa.gov

2. MOL The Mission Operations Laboratory Page 2 NASA MSFC Huntsville, Alabama General Information  PACE initial hardware delivered on 19A. Additional PACE Samples to be delivered on ULF5. Also uses existing FIR/LMM hardware.  PD: Tibor Lorik- GRC PI: William Meyer - GRC  PD/PI support ops from GRC TSC  Experiment uses the Light Microscopy Module in the Fluids Integrated Rack (LAB1S4)  Characterize LMM higher magnification capabilities (100X)  Crew setup similar to Controlled Vapor Bubble but with oil immersion (not air)  Ops conducted from ground  LMM characterization w/o ARIS (during crew sleep, crew nominal ops, crew exercise)  LMM characterization w/ ARIS (during crew sleep, crew nominal ops, crew exercise)  ARIS Calibration must be completed prior to PACE Ops

3. MOL The Mission Operations Laboratory Page 3 NASA MSFC Huntsville, Alabama General Information (cont.)  Test data will be used to evaluate the capabilities of the LMM Microscope to support follow-on science experiments  Test data is obtained from:  PACE Target (2-D static image)  PACE Particle Sample (3-D particles of various known diameters (dyed) in a water solution)  PACE Tissue Sample (fixed biological samples)

4. MOL The Mission Operations Laboratory Page 4 NASA MSFC Huntsville, Alabama PACE Hardware (1)  PACE Test Target (1)  Sapphire lens covering Air Force test target  PACE Oil Dispenser (10)  Pipettes which dispense ~ 25 μl of Type A oil (filled to 35 μl) onto the test target PACE Test Target PACE Oil Dispenser Installed onto PACE Test Target PACE Oil Dispenser

5. MOL The Mission Operations Laboratory Page 5 NASA MSFC Huntsville, Alabama PACE Hardware (2)  PACE Particle Sample & PACE Tissue Sample  Used to further characterize the optical capabilities of the LMM Microscope using high magnification  Consists of two magnets on bottom of sample which allow it to magnetically attach to the PACE Test Target  PACE Particle Sample will be mixed using the BCAT-4 Magnet prior to installation PACE Sample

6. MOL The Mission Operations Laboratory Page 6 NASA MSFC Huntsville, Alabama General PACE2 Ops Flow Replace I/O Drive (if req’d) B Rotate LMM to Service Position PACE Sample Install D B PACE2 Ops Tst Cmd Open Rack Doors PRO Activate Rack FIR Rack Power Up Rotate LMM to Operate Position PACE2 Ops Tst Cmd C C Close Rack Doors Open Rack Doors Rotate LMM to Service Position Rotate LMM to Operate Position Close Rack Doors LMM Power Up PACE Sample Reconfig Remove ARIS align guides Install ARIS align guides PRO Dectivate Rack 2 times (w/ and w/o ARIS) G D PACE2 Ops Tst Cmd PRO Activate Rack FIR Rack Power Up E F Open Rack Doors Rotate LMM to Service Position Rotate LMM to Operate Position Close Rack Doors LMM Power Up PACE Sample Reconfig PRO Dectivate Rack 4 times (w/ or w/o ARIS) Second time go to D Fourth time go to G G Open Rack Doors Rotate LMM to Service Position Rotate LMM to Operate Position Close Rack Doors Remove ARIS align guides Install ARIS align guides PACE Sample Remove PODF Crew Procedure Legend PD CommandPRO Command

7. MOL The Mission Operations Laboratory Page 7 NASA MSFC Huntsville, Alabama PACE Resource Requirements  752 watts during operations  45 kg/hr MTL flow cooling  6.5 Mbps P/L video (NRT)  400 kbps medium rate data (NRT)  Requires ISS “over-the-shoulder” video during crew tasks (NRT)

8. MOL The Mission Operations Laboratory Page 8 NASA MSFC Huntsville, Alabama PACE Crew Procedures  2.004 FCF PACE Sample Reconfiguration  Replace sample (particle or tissue)  Replace oil dispenser and re-dispense (as required)  2.005 FCF PACE Particle Sample Mixing  Use BCAT mixing magnet to mix particles.  2.006 FCF PACE Sample Installation  Remove surveillance camera  Install base, sample (particle or tissue) and oil dispenser  Dispense oil onto target  2.007 FCF PACE Sample Removal  Clean up oil on test target  Remove base, sample and oil dispenser  Re-install surveillance camera

9. MOL The Mission Operations Laboratory Page 9 NASA MSFC Huntsville, Alabama LMM Crew Procedures  2.035 FCF LMM OBJECTIVE LENS REMOVAL  Remove a lens from the LMM microscope  2.036 FCF LMM AFC FRONT DOOR REMOVAL, INSTALLATION  Remove or install the AFC front door on the LMM  2.037 FCF LMM OBJECTIVE LENS INSTALLATION  Install a lens on the LMM microscope

10. MOL The Mission Operations Laboratory Page 10 NASA MSFC Huntsville, Alabama PACE Safety Summary  PACE Test Target, LMM Microscope Lenses, & CVB Surveillance Camera  Shatterable materials  PACE Oil Dispenser  Leakage  Rated tox level 1 (Type A oil)  PACE Particle Sample and PACE Tissue Sample  Leakage

11. MOL The Mission Operations Laboratory Page 11 NASA MSFC Huntsville, Alabama PACE Acronyms AFCAuxiliary Fluids Container ARISActive Rack Isolation System CVBConstrained Vapor Bubble FCFFluids and Combustion Facility FIRFluids Integrated Rack GRCGlenn Research Center LMMLight Microscopy Module PACEPreliminary Advanced Colloids Experiment TSCTelescience Support Center

12. MOL The Mission Operations Laboratory Page 12 NASA MSFC Huntsville, Alabama PACE Test Target More details: The image on the left is an overview of the Air Force Test Target that was used for the LMM test target. The image on the right is a 100 x image of the smallest subsection of the test target. This image was taken with an oil immersion objective, coupled directly to the target face. The image was taken with the camera set for Autoexpose, which yielded an exposure time of 1.61 ms. The image gives a contrast ratio of ~ 73%, which should yield a very effective method for measuring image variability due to motion or de-focus. Air Force Test Target that was used for the Light Microscopy Module (LMM) test target Test target is shown on the left. It’s image, as seen (and inverted) by the microscope, is shown on the right. PACE Module with test target