1. MOL The Mission Operations Laboratory MOL The Mission Operations Laboratory NASA MSFC Huntsville, Alabama BRIC-17 TIR Overview Aug 28, 2012 Tony Cox EO20 Payload Ops Lead 256-961-0039 tony.w.cox@nasa.gov

2. MOL The Mission Operations Laboratory Page 2 NASA MSFC Huntsville, Alabama General Information  The Biological Research in Canisters (BRIC) system is an anodized- aluminum canister used to provide passive stowage for investigations studying the effects of space flight on small specimens.  BRIC canisters provide a sterile growth environment for biological samples, a chemical preservative mechanism, and three levels of containment for hazardous materials and autonomous temperature data logging to support studies in cell and tissue cultures or plants.  BRIC Canisters can support most any experiment that will fit into a 60mm petri dish.  BRICs have supported a wide variety of research on many Space Shuttle missions.  BRIC has ground controls which require crew real-time call down.  BRIC-17 crew training will consist of a short video detailing their hardware and operations.

3. MOL The Mission Operations Laboratory Page 3 NASA MSFC Huntsville, Alabama BRIC-17 Hardware  BRIC-17 consists of 4 BRIC Canisters, Actuator Tool and Rod Kit The BRIC canister is the crew interface for actuations by using the Actuator Tool and Rod KIt Each BRIC Canister contains 5 Petri Dish Fixation Units (PDFUs) 1 HOBO Data Logger Actuator Tool Rod Kit

4. MOL The Mission Operations Laboratory Page 4 NASA MSFC Huntsville, Alabama BRIC Actuation

5. MOL The Mission Operations Laboratory Page 5 NASA MSFC Huntsville, Alabama BRIC-17 Operations  BRIC-17 will ascend in a CTB at ambient on SpaceX-2. Requirement is 20-25°C Timeline for BRIC Actuations:  BRIC Canisters labeled A and B  This PI’s science is “activated” upon exposure to microgravity. (No crew required)  This PI requires that their science be “fixated” within a window of 10-15 days of exposure to microgravity, with an optimum fixation on the 12 th day. (using Actuator Tool/Rod)  12 to 24 hours after fixation, the canisters shall be placed into MELFI to freeze the science.   BRIC Canisters labeled C and D  This PI’s science is “activated” using Actuator Tool/Rod. There is no requirement as to what day this must be done  This PI requires that their science be “fixated” anytime from 7 to 10 days after activation (optimum day 8)  12 to 24 hours after fixation, the canisters shall be placed into MELFI to freeze the science.  BRIC-17 will return in DCB at -20C or below

6. MOL The Mission Operations Laboratory Page 6 NASA MSFC Huntsville, Alabama Safety Summary  The Safety Data Pack …….has not yet been signed. However, there are no expected hazards for this experiment.  PD may request a “keep away from heat source” Payload Reg. (heat source may be detrimental to the science – not a safety concern to crew)  BRIC Canisters provide three levels of containment.

7. MOL The Mission Operations Laboratory Page 7 NASA MSFC Huntsville, Alabama Back up Slide – PDFU Actuation

8. MOL The Mission Operations Laboratory Page 8 NASA MSFC Huntsville, Alabama Back up Slide – BRIC Assembly

9. MOL The Mission Operations Laboratory Page 9 NASA MSFC Huntsville, Alabama BRIC-17 Arrival/Crossover to ISS Destow BRIC-17 Payload Activate Canisters C& D Fix Canisters A & B Earliest start time: Arrival at ISS Latest start time: Arrival at ISS+ 168 hrs Optimal start time: None specified Earliest start time: Launch+ 192 hrs Latest start time: Launch+ 360 hrs Optimal start time: Launch+ 288 hrs Fix Canisters C & D Earliest start time: Activation+168 hrs Latest start time: Activation+240 hrs Optimal start time: Activation+192 hrs Minimum Dwell Time: 12 hours Maximum Dwell Time: 24 hours Optimal Dwell Time: 12 hours Launch/Enter Microgravity Dwell Post-Fixation for each Canister (A,B,C, &D) Pre-Insertion into MELFI Insert Canisters A,B, C & D into MELFI Transfer Canisters A,B, C & D from MELFI to Cold Bag Actuation Dependencies Dragon Capsule Return/BRIC-17 Turnover to KSC

10. MOL The Mission Operations Laboratory Page 10 NASA MSFC Huntsville, Alabama BRIC-17 BRIC-17 has a total of 4 BRIC canisters containing two different experiments – One experiment contained in canisters A and B; the second is contained within C and D – Experiment in canisters A and B only requires one crew interaction (fixation) – Experiment in canisters C and D requires two crew interactions (activation & fixation) Actuation dependencies vary between the two experiments – Canisters A and B self-activate upon launch and entry into microgravity – Canisters C and D must be actuated by the crew post-arrival at the ISS There is no requirement to synchronize actuation of the two experiments. However, there may be operational efficiencies in doing so: – Best opportunity seem to be fixation of canisters A, B, C and D and remaining downstream activities – Earliest start, latest start and optimal start timeframes have been provided for each activity