1. Surprise quiz for UG 5 semester I (session 2012-16) ASAB T.A.Baig

2. 1. The rate-determining enzyme of glycolysis: 2. The rate-determing enzyme of gluconeogenesis: 3. The rate-determining enzyme of the citric acid cycle: 4. What are the 5 cofactors of pyruvate dehydrogenase complex 5. A deficiency in this vitamin leads to the inability to oxidize pyruvate and thus has a major neurological impact (Beriberi)

3. 6. Hexokinase: Substrate 7. Hexokinase: Product 8. Hexokinase: Inhibitors 9. Glucokinase: Found in what type of cells 10. PFK-1: Location

4. 11. PFK-1: Product 12. PFK-1: Substrate 13. PFK-1: Inhibitors 14. PFK-1: Activators 15. Pyruvate Dehydrogenase (PDHC): Product

5. 16. Net result of glycolysis 17. In the skeletal muscle and heart, what are the two possible fates of glucose? 18. How many ATPs produced from aerobic glycolysis? 19. In anaerobic glycolysis, how many ATPs are used? 20. What occurs if there is a deficiency in GLUT?

6. ANSWERS

7. 1. The rate-determining enzyme of glycolysis: phosphofructokinase-1 (PFK-1) 2. The rate-determing enzyme of gluconeogenesis: The rate-determing enzyme of gluconeogenesis: 3. The rate-determining enzyme of the citric acid cycle: isocitrate dehydrogenase 4. What are the 5 cofactors of pyruvate dehydrogenase complex Thiamine pyrophosphate [TPP (B1)] Lipoic acid FAD (riboflavin) NAD+ (niacin) Coenzyme A (pantothenic acid) 5. A deficiency in this vitamin leads to the inability to oxidize pyruvate and thus has a major neurological impact (Beriberi) Thiamine (B1) 6. Hexokinase: Substrate Glucose 7. Hexokinase: Product Glucose-6-P 8. Hexokinase: Inhibitors Glucose-6-P 9. Glucokinase: Found in what type of cells Liver & Pancrease

8. 10. PFK-1: Location Cytosol 11. PFK-1: Product Fructose-1,6-Bisphosphate 12. PFK-1: Substrate Fructose-6-P 13. PFK-1: Inhibitors ATP, Citrate 14. PFK-1: Activators AMP, Fructose-2,6-Bisphosphate (most powerful) 15. Pyruvate Dehydrogenase (PDHC): Product Acetyl-CoA 16. Net result of glycolysis 2 Pyruvate 2 cytoplasmic NADH 4 ATP - 2 ATP invested = 2 ATP 17. In the skeletal muscle and heart, what are the two possible fates of glucose? It can be completely oxidized or it can be stored as glycogen. 18. How many ATPs produced from aerobic glycolysis? 10 19. In anaerobic glycolysis, how many ATPs are used? 6 20. What occurs if there is a deficiency in GLUT? It may lead to diabetes

9. Aaye guzrey baras Bata tjhy bhoolun kesy…? Tery Lamhon ne mry Brso’n ki rfaqat cheeni