1. Inc 23/24 Integrated Team Review Intravenous Fluid Generation (IV Gen)

2. Science Overview With NASA’s desire to return to the Moon and to extend manned exploration missions to Mars, there is an increased likelihood of a mishap and/or medical emergency. With a focus on minimizing up-mass for exploration missions, it is desirable to have a system onboard that will produce intravenous (IV) solutions as needed rather than flying large quantities of pre-made IV solutions that have a limited shelf life. IV Gen will demonstrate a prototype system to produce Sterile Water for Injection (SWI) in a reduced gravity environment. After purification, the water is mixed with NaCl to produce a normal saline solution. This solution will be returned to Earth for testing. Fluid physics data will allow for scalability of filters and IV fluid production rate to meet exploration requirements.

3. Payload Overview –Hardware launches on 19A –Operates inside the Microgravity Science Glovebox –Operations performed close to the docking of the next flight –Two saline bags will be returned on ULF4 –Up to 4 additional bags of purified water will be generated to exhaust the filter which will provide data for future scaling –5 major hardware pieces Accumulator, Purifier, Mixer, Data Acquisition and Control Unit, and Power Converter –Input water is from ISS potable water source Water Processing Assembly (WPA) Contingency Water Container – Iodine (CWC-Iodine) –GN2 is used to collapse the inner bag of the Accumulator which pushes water into the Purifier. –Four bags of purified water will be reclaimed into the ISS atmosphere –Three MSG Video Cameras will be installed to record experiment runs –SAMS TSH will be installed for acceleration measurement data

4. Hardware Overview SAMS TSH Power Converter Data Acquisition and Control Unit Mixer Accumulator Purifier

5. ISS Potable Water Contingency Water Container - Iodine LAB1D4 (WRS-1) Potable Water Manual Container QD (RIP) WPA H2O Transfer Common Hose

6. Accumulator ¼” thick Lexan housing for observation Approximately 1.5 liter capacity Uses GN2 to push potable water to the Purifier Custom polyurethane bladder Secured by Velcro to base plate No electrical power dependency Double shut-off quick disconnect valves Water inlet from WPA or CWC-I Water outlet to Purifier GN2 inlet from MSG Accumulator Housing with internal polyurethane bladder

7. Purifier Deionizing resin and membrane filters to purify the water Flow meter to measure the flow Pressure transducers to monitor the pressure at different points during the purification process Conductivity sensors to compare water conductance before and after deionization Clear Lexan cover for video camera imaging Left two blue 2.0 Pall filters Conductivity sensor after DI resin cartridge White.2 micron filters Access door to activate filter by- pass in case of premature blockage (off-nominal) Top view

8. Access Door (off nominal use) Potable Water Shutoff Valve Captive fasteners Deionizing Resin Valves Power Data Purifier IV Fluid Outlet Saline Inlet Purified Water Outlet 1.2 µm Air Elimination Filters Conductivity Sensors

9. Holds two 2-Liter bags via Velcro Teflon-coated stir bar and sodium crystals pre-positioned in Saline bag Saline bag placed on plate with magnetized motor Manual capability for crew control of motor Rotor is visible to allow for stir bar positioning. –Pull force = 1 lb/magnet –900 rpm max Once mixing has been initiated by the crew, it will be software controlled Mixing speed and duration are uplink parameters Homogenizes solution within 20 minutes Mixer Motor Speed Controller Mixer Manual On/Off Electrical Connector

10. Collection Bag Saline Bag Stir bar NaCl Pressure Cuff (Saline Bag installed ) Bag to Bag transfer will check conductivity to ensure homogenous solution Squeeze solution from Saline Bag into a Collection Bag using a Pressure Cuff (works similar to a blood pressure cuff) Collection Bag has sterility filters Diameter of the stir bar is larger than diameter of tubing

11. Converts Power from MSG (J116 on the left side) Supplies +28Vdc to the Data Acquisition and Control Unit (J117) Supplies +24Vdc to the Purifier (J118) Power Converter

12. LEDs Data Acquisition and Control Unit Provides data handling, data storage, and software control functions for the different IV Gen sub-systems. J111 connects to the Power Converter J114 connects to the MSG J404 Data Feedthrough to pass data to the MLC J113 connects to the MSG RS-422 on the rear wall for low rate telemetry and payload commanding J112 connects to the Purifer and Mixer for command and control

13. Operations Overview Ground rules and constraints –Once an IV Gen WPA Hose has been used, it must be replaced after 72 hours –Samples generated from Saline Operations will be returned on ULF4 –Samples generated from Purified Water Operations will be reclaimed in the ISS atmosphere –Saline Ops and Purified Water Ops will occur on consecutive days to prevent bacteria growth in the filters Run 3-6 Run 1 & 2 Saline Operations N004 Purified Water Ops N005 Hardware Setup N001 GN2 Leak Test N002 Accumulator Fill N003 After Run 6 Hardware Stow N007 Video tape Exchange N006

14. Hazard Report Number Hazard DescriptionCntrl Num Hazard Control STD-IVGEN-01Mating/demating powered connectors 14.b.3Confirm power has been removed from MSG prior to mating/demating connectors. IVGEN-02Leakage/Inadvertent Release of Fluid 4.2Visually check collection bags, Purifier, and tubing for damage and/or leakage before, during, and after use. IVGEN-02Leakage/Inadvertent Release of Fluid 6.1 Instruct on techniques (valve sequencing and tube clamping) to minimize or eliminate spillage during, assembly, operation, and disassembly of IV Gen hardware. IVGEN-02Leakage/Inadvertent Release of Fluid 6.2 Provide cleaning tools/wipes in case of spillage IVGEN-03Rupture or Leakage of Pressurized System 1.2Perform a leak test on the IV Gen/MSG QD interface. IVGEN-03Rupture or Leakage of Pressurized System 1.3Close the MSG GN2 needle valve prior to mating/demating all GN2 QDs. IVGEN-04Inspect Saline Bags for damage prior to removing from protective packaging. MSG-Int-IV GEN-02Unintended Reactions of Chemicals in the MSG Filters 1.3If a leak should occur, the flight crew will consult MSG staff on console for instructions concerning whether to change any of the air filters in the MSG facility and whether to continue any of the experiments. MSG-Int-IV GEN-03Crew Exposure to Asphyxiating Environment 1.1A pressure decay test will be performed after IV Gen setup in the WV to ensure no leakage of GN2 exists before operation. MSG-Int-IV GEN-03Crew Exposure to Asphyxiating Environment 1.2Purge time of two minutes in OPEN mode before a crew member can access the MSG WV through an access port.

15. IV Gen Hardware Setup in the MSG

16. IV Gen Complete Hardware Setup with Saline Bag installed

17. IV Gen hardware in MSG with Pressure Cuff

18. CWC –Iodine Compatible Water Container – Iodine IV Gen Intravenous Fluid Generation MSGMicrogravity Science Glovebox NaClSodium Chloride RIP Rack Interface Panel SAMS Space Acceleration Measurement System SWISterile Water for Injection TSH Triaxial Sensor Head USP United States Pharmacopoeia WPAWater Processing Assembly WRS Water Recovery System ACRONYMS