1. LAB FOUR Photosynthesis

2. 4A Plant Pigment Chromatography 4B Tracking the Light Reaction LAB FOUR Photosynthesis

3. How is the light reaction affected by the presence or absence of light? How is it affected by the boiling of chloroplasts?

4. Isolated chloroplasts LAB FOUR Photosynthesis

5. Isolated chloroplasts LAB FOUR Photosynthesis

6. boiled unboiled LAB FOUR Photosynthesis Isolated chloroplasts

7. boiled unboiled LAB FOUR Photosynthesis Isolated chloroplasts

8. boiled unboiled LAB FOUR Photosynthesis Isolated chloroplasts light dark

9. LAB FOUR Photosynthesis Isolated chloroplasts DPIP

10. LAB FOUR Photosynthesis Isolated chloroplasts DPIP

11. LAB FOUR Photosynthesis Isolated chloroplasts boiled unboiled boiled unboiled

12. LAB FOUR Photosynthesis Isolated chloroplasts boiled unboiled boiled unboiled blank

13. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis.

14. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless.

15. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 0%

16. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 10%

17. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 40%

18. LAB FOUR Photosynthesis Isolated chloroplasts DPIP replaces NADP+ in the light reactions of photosynthesis. As DPIP is reduced, it turns from blue to colorless. A spectrophotometer measures the absorption of blue light. 80%

19. Cuvettes: 1.Blank (H 2 O only) 2.In the dark 3.Normal 4.Boiled chloroplasts 5.No chloroplasts LAB FOUR Photosynthesis

20. 12345 LightYesNoYes Buffer1 mL H2OH2O4 mL3 mL 3 mL + 3 drops DPIP1 mL Chloro- plasts 3 drops 3 drops boiled No Cuvettes: Keep all cuvettes on ice!

21. Adjust empty spectrophotometer to 0% Adjust with blank cuvette to 100% Add 3 drops chloroplast to your cuvette and immediately measure transmittance. Measure again at 5, 10, and 15 minutes. LAB FOUR Photosynthesis

22. Five teams will handle one cuvette each Each team will take four measurements Your turn lasts exactly one minute. LAB FOUR Photosynthesis

23. 12345 LightYesNoYes Buffer1 mL H2OH2O4 mL3 mL 3 mL + 3 drops DPIP1 mL Chloro- plasts 3 drops 3 drops boiled No Cuvettes: Keep all cuvettes on ice!.

24. Adjust empty spectrophotometer to 0% Adjust with blank cuvette to 100% Add 3 drops chloroplast to your cuvette and immediately measure transmittance. Measure again at 5, 10, and 15 minutes.. LAB FOUR Photosynthesis