1. Ch. 19 Warm-up 1. Why do many scientists classify viruses as non-living? 2. Draw the basic structure of a virus. Label and define capsid, viral envelope and nucleic acid.

2. Ch. 19 Warm-up 1. Draw the lytic/lysogenic cycle. 2. What stage of the lytic-lysogenic cycle is a virus virulent? Temperate? 3. What determines a host range?

3. Viruses Chapter 19

4. What you must know: The components of a virus. The differences between lytic and lysogenic cycles. How viruses can introduce genetic variation into host organisms. Mechanisms that introduce genetic variation into viral populations.

5. Bacteria vs. Viruses BacteriaVirus Prokaryotic cell Most are free-living (some parasitic) Relatively large size Antibiotics used to kill bacteria Not a living cell (genes packaged in protein shell) Intracellular parasite 1/1000 size of bacteria Vaccines used to prevent viral infection Antiviral treatment

6. Viruses Very small (<ribosomes) Components = nucleic acid + capsid ◦ Nucleic acid: DNA or RNA (double or single-stranded) ◦ Capsid: protein shell ◦ Some viruses also have viral envelopes that surround capsid

7. Viruses Limited host range ◦ Entry = attach to host cell membrane receptors through capsid proteins or glycoproteins on viral envelope (animal) ◦ Eg. human cold virus (rhinovirus)  upper respiratory tract (mouth & nose) Reproduce quickly within host cells Can mutate easily ◦ RNA viruses: no error-checking mechanisms

11. Simplified viral replicative cycle

12. VIDEO: T4 PHAGE INFECTION T4 PHAGE INFECTIONT4 PHAGE INFECTION

13. Viral Reproduction Lytic Cycle: Lytic Cycle: ◦ Use host machinery to replicate, assemble, and release copies of virus ◦ Virulent phageslysisapoptosis ◦ Virulent phages: Cells die through lysis or apoptosis Lysogenic (Latent) Cycle: Lysogenic (Latent) Cycle: ◦ DNA incorporated into host DNA and replicated along with it prophage ◦ Bacteriophage DNA = prophage provirus ◦ Animal virus DNA = provirus ◦ UV radiation, chemicals: lysogenic  lytic cycle ◦ Temperate Phage ◦ Temperate Phage: uses both methods of replication

14. Bacteriophage Virus that infects bacterial cells

15. Lytic Cycle of T4 Phage

16. Lytic Cycle vs. Lysogenic Cycle

17. Animal viruses have a membranous envelope Host membrane forms around exiting virus Difficult for host immune system to detect virus

18. VIDEO: HOW DENGUE VIRUS ENTERS A CELL HOW DENGUE VIRUS ENTERS A CELLHOW DENGUE VIRUS ENTERS A CELL

19. VIDEO: HIV LIFE CYCLE HIV LIFE CYCLEHIV LIFE CYCLE

20. Retrovirus reverse transcriptase RNA virus that uses reverse transcriptase (RNA  DNA) provirus Newly made viral DNA inserted into chromosome of host (provirus) Host transcribes provirus to make new virus parts Example: HIV (Human Immunodeficiency Virus) Example: HIV (Human Immunodeficiency Virus)

21. HIV = Retrovirus

22. ◦ Origin: Chimpanzee virus ◦ Infects white blood cells (helper T) ◦ HIV+: provirus (DNA inserted), latent ◦ AIDS: <200 WBC count, opportunistic infections HIV

23. Other Human Viruses Herpes virus Smallpox Herpes Simplex Virus 1 (HSV-1) Herpes Simplex Virus 2 (HSV-2) Eradicated in 1979 due to worldwide vaccination campaigns

24. Emerging Viruses Emerging Viruses = mutation of existing viruses Pandemic: global epidemic

25. Current Outbreaks Zika Virus ◦ Spread by Aedes mosquitoes (Aedes aegypti) ◦ Major outbreak in Brazil and Latin America ◦ Linked to birth defects (microcephaly) Dengue Fever Chikungunya

26. Zika Virus (as of Dec. 2015)

27. Drugs for Prevention/Treatment Vaccine: weakened virus or part of pathogen that triggers immune system response to prevent infection ◦ Ex. HPV, MMR, HepA, Flu shot Antiviral Drugs: block viral replication after infection ◦ Ex. Tamiflu (influenza), AZT (HIV)

29. Viroids Small, circular RNA molecules that infect plants Cause errors in regulatory systems that control plant growth Eg. coconut palms in Philippines

30. Prions Misfolded, infectious proteins that cause misfolding of normal proteins Eg. scrapie (sheep), mad cow disease (BSE), Creutzfeldt-Jakob disease (humans), kuru (humans – New Guinea)

31. Diseases caused by prions Prions act slowly – incubation period of at least 10 years before symptoms develop Prions are virtually indestructible (cannot be denatured by heating) No known cure for prion diseases Kuru in New Guinea

32. Prion Neurodegenerative Diseases Alzheimer’s Disease Parkinson’s Disease

33. AMOEBA SISTERS: VIRUSES: VIRUS REPLICATION AND THE MYSTERIOUS COMMON COLD VIRUSES: VIRUS REPLICATION AND THE MYSTERIOUS COMMON COLDVIRUSES: VIRUS REPLICATION AND THE MYSTERIOUS COMMON COLD

34. TED-ED: HOW WE CONQUERED THE DEADLY SMALLPOX VIRUS HOW WE CONQUERED THE DEADLY SMALLPOX VIRUSHOW WE CONQUERED THE DEADLY SMALLPOX VIRUS