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Air Pollution as a Research Topic Lea Serres
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Need Human longevity increases with cleaner air (Pope, 2009) Ozone pollution greater now(Wiley- Blackwell 2008)
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Forced Expiratory Volume After Exposure to Nitrogen Dioxide (Gauderman, 2004)
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Negative Effects 20 percent tree reduction in 2100 (Wiley – Blackwell 2008) Tree density on a Gradual Decline
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Decrease in Mean Density of Trees (Carr, 2008)
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Organisms at Risk Due to Air Pollution Air pollution affects other organisms than humans Birds, amphibians, and coral also affected (Wiley- Blackwell 2008)
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Organisms at Risk Due to Air Pollution Wiley- Blackwell, 2008)
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Financial Effects 15 billion dollars spent on environmental investments in 2000 (Michaels, 2007) Cost of air pollution continually rising since 1980’s (Castagno, 2005)
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Human Effects Negative effects on human lungs (Kelly, 2008) People with respiratory disease experience worsening symptoms (Kelly, 2008)
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Increased Lung Cancer Risk 1800 men exposed to moderate air pollution (Begany, 2006) 418 developed lung cancer (Begany, 2006)
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Dr. Singh Composition and chemistry of atmosphere Prominent field- chemical engineering Goal- understand human impact on environment
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Dr. Guaderman Working on determining health outcomes of environmental exposures Works with Children’s health studies
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Nitrogen Dioxide Studies Inoue, Fukunaga, Okubo Test- lethal properties, mutagenic effects Model organism- Drosophila melanogaster
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Drosophila Melanogaster Short lifespan, cheap, small Sexing, and synchronization necessary
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Purpose The purpose of this experiment would be to test the effects that Nitrogen Dioxide has on the longevity of Drosophila melanogaster in progressive concentrations.
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Hypothesis Alternative Hypothesis- Increased levels of nitrogen dioxide would have an effect on the longevity of the model organism, Drosophila melanogaster
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Methodology Control Group- No Nitrogen Dioxide (N=60) V1- 10 mg Nitrogen Dioxide (N=60) V2- 20 mg Nitrogen Dioxide (N=60) V3- 30 mg Nitrogen Dioxide (N=60) V4- 40 mg Nitrogen Dioxide (N=60) V5- 50 mg Nitrogen Dioxide (N=60) Fed Artificial Drosophila Medium Sexed and Synchronized Daily Male- dead counted for longevity data Female- dead counted for longevity data Data graphed and analyzed
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