1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Human Anatomy & Physiology, Sixth Edition Elaine N. Marieb PowerPoint ® Lecture Slides prepared by Vince Austin, University of Kentucky 3 Cells: The Living Units Part D

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Why Would a Cell Divide? As cells absorb nutrients and get larger, the volume of the cell increases faster than the surface area This means that a cell can no longer absorb nutrients and get rid of wastes fast enough to support its demands (volume) So what’s a cell to do? DIVIDE !!!!

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Why Would a Cell Divide? Besides growth a cell would also divide for: Repair or Replacement Cancer Different cells divide at different rates: Most mammalian cells = 12-24 hours Some bacterial cells = 20-30 minutes

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Getting Older… All cells are only allowed to complete a certain number of divisions Then they die (programmed cell death) How does cell division change over a lifetime? Childhood = cell division > cell death Adulthood = cell division = cell death The Later Years = cell division < cell death

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Cell Cycle Tidbits How long is one cell cycle? Depends on the cell- skin cells = ~24 hours, nerve cells = never after maturity, cancer cells = very short Remember: every cell only has a certain # of divisions it can undergo, then it dies = apoptosis (programmed cell death)

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings The Cell Cycle

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Stages of the Cell Cycle interphase (growth & replication of DNA) mitotic phase (division of cell into 2 daughter cells) Cell spends about 90% of the time in interphase There are two stages to a cells life.

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Interphase Divided into 3 phases: G1 (1 st gap) = small cell is absorbing nutrients, growing & doing its job. S (synthesis) = cell is continuing to grow & duplicates its DNA. G2 (2 nd gap) = cell keeps growing & doing its job.

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Mitosis: A Closer Look DNA is all twisted up into a Chromosome. During S phase the Chromosome is copied. 2 complete identical sets of chromosomes. They are connected in the middle by a centromere. A single copied chromosome is called a Chromatid.

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication DNA helices begin unwinding from the nucleosomes Helicase untwists the double helix and exposes complementary strands The site of replication is the replication bubble Each nucleotide strand serves as a template for building a new complementary strand

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication The replisome uses RNA primers to begin DNA synthesis DNA polymerase III continues from the primer and covalently adds complementary nucleotides to the template DNA Replication PLAY

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication Since DNA polymerase only works in one direction: A continuous leading strand is synthesized A discontinuous lagging strand is synthesized DNA ligase splices together the short segments of the discontinuous strand Two new telomeres are also synthesized This process is called semiconservative replication

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication Figure 3.31

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Cell Division Essential for body growth and tissue repair Mitosis – nuclear division Cytokinesis – division of the cytoplasm

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings The Mitotic Phase Divided into 4 stages of Mitosis: Prophase Metaphase Anaphase Telophase (+) PLUS Cytokinesis

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Early and Late Prophase Asters are seen as chromatin condenses into chromosomes Nucleoli disappear Centriole pairs separate and the mitotic spindle is formed Prometaphase PLAY Prophase PLAY

17. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Early Prophase Figure 3.32.2 Early prophase Early mitotic spindle Pair of centrioles Centromere Aster Chromosome, consisting of two sister chromatids

18. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Late prophase Fragments of nuclear envelope Polar microtubules Kinetochore Kinetochore microtubule Spindle pole Late Prophase Figure 3.32.2

19. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Metaphase Chromosomes cluster at the middle of the cell with their centromeres aligned at the exact center, or equator, of the cell This arrangement of chromosomes along a plane midway between the poles is called the metaphase plate Metaphase PLAY

20. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Metaphase Figure 3.32.4

21. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Anaphase Centromeres of the chromosomes split Motor proteins in kinetochores pull chromosomes toward poles Anaphase PLAY

22. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Daughter chromosomes Anaphase Figure 3.32.5 Anaphase

23. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Telophase and Cytokinesis New sets of chromosomes extend into chromatin New nuclear membrane is formed from the rough ER Nucleoli reappear Generally cytokinesis completes cell division Telophase PLAY

24. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Telophase and Cytokinesis Figure 3.32.5 Telophase and cytokinesis Nucleolus forming Contractile ring at cleavage furrow Nuclear envelope forming

25. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Cytokinesis Cleavage furrow formed in late anaphase by contractile ring Cytoplasm is pinched into two parts after mitosis ends

26. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Protein Synthesis DNA serves as master blueprint for protein synthesis Genes are segments of DNA carrying instructions for a polypeptide chain Triplets of nucleotide bases form the genetic library Each triplet specifies coding for an amino acid

27. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings From DNA to Protein Figure 3.34

28. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Roles of the Three Types of RNA Messenger RNA (mRNA) carries the genetic information from DNA in the nucleus to the ribosomes in the cytoplasm Transfer RNAs (tRNAs) bound to amino acids base pair with the codons of mRNA at the ribosome to begin the process of protein synthesis Ribosomal RNA (rRNA) is a structural component of ribosomes

29. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Transcription Transfer of information from the sense strand of DNA to RNA Transcription factor Loosens histones from DNA in the area to be transcribed Binds to promoter, a DNA sequence specifying the start site of RNA synthesis Mediates the binding of RNA polymerase to promoter

30. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Transcription: RNA Polymerase An enzyme that oversees the synthesis of RNA Unwinds the DNA template Adds complementary ribonucleoside triphosphates on the DNA template Joins these RNA nucleotides together Encodes a termination signal to stop transcription

31. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Overview of Transcription Figure 3.35

32. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Initiation of Translation A leader sequence on mRNA attaches to the small subunit of the ribosome Methionine-charged initiator tRNA binds to the small subunit The large ribosomal unit now binds to this complex forming a functional ribosome

33. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Polypeptide Chain Elongation Figure 3.37

34. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Genetic Code RNA codons code for amino acids according to a genetic code Figure 3.36

35. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Information Transfer from DNA to RNA DNA triplets are transcribed into mRNA codons by RNA polymerase Codons base pair with tRNA anticodons at the ribosomes Amino acids are peptide bonded at the ribosomes to form polypeptide chains Start and stop codons are used in initiating and ending translation

36. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Information Transfer from DNA to RNA Figure 3.39

37. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Developmental Aspects of Cells All cells of the body contain the same DNA but develop into all the specialized cells of the body Cells in various parts of the embryo are exposed to different chemical signals that channel them into specific developmental pathways Genes of specific cells are turned on or off Cell specialization is determined by the kind of proteins that are made in that cell

38. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Developmental Aspects of Cells Development of specific and distinctive features in cells is called cell differentiation Cell aging Wear and tear theory attributes aging to little chemical insults and formation of free radicals that have cumulative effects throughout life Genetic theory attributes aging to cessation of mitosis that is programmed into our genes