1. Soybean SE Transformation I.Induction Plant and zygotic embryo production (6-8 weeks) Induction on D40 (6-8 weeks) Proliferation on solid D20 (4 weeks) Proliferation on FNL (4 weeks)

2. Soybean SE Transformation- cont. II. Gene Transfer, Selection, Proliferation & Maturation Place 20-25 SE clumps on D20 Shoot Continue on D20 (1 week) Transfer into FNL/H20 for seln. (7–10 weeks) Isolate green, well formed globular SEs, grow on multi-well plate (5 wks) Proliferate selected material in FNL (5 weeks) Mature healthy green embryos matured on SHaM (4-5 weeks) Desiccation (5-7 days)Select healthy matured SEs for lipid and/or other analysis

3. Soybean SE Transformation- cont. III. Conversion & Seed Production Desiccated SEs Germination (1 week) Plant hardening (3 weeks) Transfer hardy plantlets into pots, grow-out in greenhouse Harvest mature seeds (10-16 weeks)