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Published byJoan Bruce Modified over 9 years ago
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SYBR Green I Chemistry Detects all double stranded DNA
Low cost alternative can easily be upgraded to TaqMan assay Dissociation curves needed PCR, Light
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Smarter chemistry: The TaqMan™ probe
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TaqMan® chemistry R Q TaqMan® Probe Forward Primer Reverse Primer 5’ p
3’ 5’ 5’ 3’ 5’ Reverse Primer
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TaqMan® chemistry R Q 5’ p 3’ 5’ 5’ 3’ 5’ Displacement and cleavage
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TaqMan® chemistry R Q 5’ p 3’ 5’ 5’ 3’ 5’ Displacement and cleavage
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TaqMan® chemistry Polymerization Strand displacement Cleavage
R = Reporter Forward Q Q = Quencher Primer R Probe 5' p 3' 3' 5' 5' 3' 5' Reverse Primer Strand displacement R Q 5' p 3' 3' 5' 5' 3' 5' Cleavage R Q 5' p 3' 3' 5' 5' 3' 5' Polymerization completed R Q p 3' 5' 3' 5' 5' 3' 5'
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Amplification plot
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Real-time versus end-point
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Closed Tube Detection Fluorescence detection through tube
Detection during amplification No contamination No further steps needed Automated Quantitation after run
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