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Flexible Tools for Gene Expression and Silencing in Tomato by Ana I. Fernandez, Nicolas Viron, Moftah Alhagdow, Mansour Karimi, Matthew Jones, Ziva Amsellem,

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Presentation on theme: "Flexible Tools for Gene Expression and Silencing in Tomato by Ana I. Fernandez, Nicolas Viron, Moftah Alhagdow, Mansour Karimi, Matthew Jones, Ziva Amsellem,"— Presentation transcript:

1 Flexible Tools for Gene Expression and Silencing in Tomato by Ana I. Fernandez, Nicolas Viron, Moftah Alhagdow, Mansour Karimi, Matthew Jones, Ziva Amsellem, Adrien Sicard, Anna Czerednik, Gerco Angenent, Donald Grierson, Sean May, Graham Seymour, Yuval Eshed, Martine Lemaire-Chamley, Christophe Rothan, and Pierre Hilson Plantphysiol Volume 151(4):1729-1740 December 4, 2009 ©2009 by American Society of Plant Biologists

2 Assemblage of different genetic elements captured into entry clones via LR reaction to facilitate genetic studies in tomato. Fernandez A et al. Plantphysiol 2009;151:1729-1740 ©2009 by American Society of Plant Biologists

3 GUS activity in tomato (cv MicroTom) stably transformed with promoter:GUS transgenes generated via MultiSite Gateway cloning. Fernandez A et al. Plantphysiol 2009;151:1729-1740 ©2009 by American Society of Plant Biologists

4 Detection of nuclear GFP in cells of tomato fruit pericarp. Fernandez A et al. Plantphysiol 2009;151:1729-1740 ©2009 by American Society of Plant Biologists

5 Tissue-specific amiRNA silencing in tomato fruits. Fernandez A et al. Plantphysiol 2009;151:1729-1740 ©2009 by American Society of Plant Biologists

6 Tissue-specific transactivation in tomato with selected Arabidopsis promoters. Fernandez A et al. Plantphysiol 2009;151:1729-1740 ©2009 by American Society of Plant Biologists

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