1. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles HRP Increment 33/34 Overview Increment Manager Kristen Kinder/NASA Increment Lead Gina Miller/LM Operations Lead Andres Ovalles/LM Export Classification: EAR99

2. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 2 HRP Increment 33/34 Team NASA Increment Manager Kristen Kinder 281-483-3224 kristen.kinder-1@nasa.gov Increment Lead Gina Miller 281-218-3016 gina.miller@lmco.com Increment Scientist Scott J. Wood 281-483-7294 scott.j.wood@nasa.gov Increment Planner Joe Coen 281-218-3211 joseph.coen@lmco.com Increment Operations Lead Andres Ovalles 281-218-3039 andres.ovalles@lmco.com Increment Training Coordinator Joyce Schultz 281-218-3051 joyce.e.schultz@lmco.com Increment Science Coordinator Alonzo Webb (32S crew) 281-218-3066 alonzo.a.webb@nasa.gov Shanna Rodgers (33S crew) 281/218-3068 shanna.rodgers@lmco.com

3. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 3 AGENDA HRP Payload Complement Crew Participation Matrix New HRP Payload Overview New Experiment Information Facility Operations Support to IP Activities Assumptions/Open Work Back up – Legacy HRP Experiment Overviews

4. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 4 HRP Inc. 33/34 Complement ExperimentsPrevious Increments Bisphosphonates (Control*)18 – 31/32 Integrated Cardiovascular19/20 – 31/32 Journals29/30 – 31/32 Nutrition 14 – 31/32 Pro K 21/22 – 31/32 Reaction Self Test21/22 – 31/32 Repository16 – 31/32 Spinal UltrasoundNew Sprint27/28 – 31/32 Treadmill Kinematics27/28 – 31/32 VO2Max19/20 – 31/32 Pre/Post Only Functional Task Test21/22 – 31/32 Hip QCT31/32 Intervertebral Disc DamageNew Manual ControlNew *Control subjects, not requiring in-flight pill ingestion, begins in Inc. 31/32

5. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 5 Investigation31S (JAXA)31S (NASA)32S (NASA)33S (CSA)33S (NASA) Bisphosphonates (control) Yes Integrated Cardiovascular 2 Sessions FD135 & R-15 2 Sessions FD135 & R-15 Operator 3 Sessions FD14, 30, 75 Journals Yes Pro K / Nutrition / Repository 1 Sessions FD120 1 Sessions FD120 5 Sessions FD15,30,60,120, R-15 (No PROK) 4 Sessions FD15, 30, 60, 120 (No Nutrition) 4 Sessions FD15, 30, 60, 120 Reaction Self Test Yes Spinal Ultrasound Operator 2 Sessions FD30 & 90 2 Sessions FD30 & 90 Sprint (Leg Scan) 2 Sessions FD120 & R-15 2 Sessions FD120 & R-15 Sprint (VO2) 3 Sessions FD 105, 135, R-15 Treadmill Kinematics 3 Sessions FD 105, 135, 165 VO2max 3 Sessions FD 105, 135, R-15 Crew Participation Matrix

6. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 6 Spinal Ultrasound –33S crew are the first subjects –Scans at FD 30 (+/-15), FD 90 (+/-15), FD 150 (+/-15) –Similar to other Ultrasound experiments (ICV Resting-Echo and Sprint) –CMRS use in COL –Software Review –PI Crew Conference –Remote guided scan focusing on images of lumbar and cervical spine Spinal Ultrasound

7. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 7 Spinal Ultrasound – Software Review

8. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 8 Sprint VO2 will start in Inc 31/32 Sprint VO2 data collections utilize a different exercise protocol than VO2max to obtain the “maximum oxygen uptake” value –VO2max uses the “extended PFE” which consists of the 2 minute rest period, three 5-minute stages at pre-determined workloads, and then 1- minute “max stages” until the subject cannot continue  This protocol took anywhere from 15-23 minutes to complete –Sprint VO2 uses a shorter protocol to get to the maximum exertion point, a 3-minute active warmup at a low workload, followed by 1-minute stages where the workload increases 25W each stage until max is reached  This protocol can last anywhere from 8-20 minutes Sprint VO2 vs. VO2max

9. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 9 Sprint VO2 will also not be collecting the same types of data compared to VO2max, resulting in the need for less peripheral Portable PFS hardware –Hardware not required for Sprint VO2:  Portable PFS Respiratory Valve Unit (RVU)  Portable PFS Turbine Flowmeter  VO2max Subject Kit –Portable PFS TFM Insert & Portable PFS 4L Rebreathing Bag  Portable PFS BP Cuff –Thermolab will not be collected as part of Sprint VO2, so none of that hardware will be used either Sprint VO2 vs. VO2max

10. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 10 HRP Inc. 33/34 Facility Activities HRF Build 12 Integrated Load Software Update –RIC v 8.3 Install GDS/PPFS Gauge Photos HRF Supply Kit Activities –Resupply –Inventory PFS Relief Valve Checkout PFS Software Update for BP-Reg PFS Software Update for RVU/ACMS ( double booked Inc 31/32 ) PuFF Syringe Maintenance HRF Rack Stowage Configuration HRF Rack 2 Troubleshooting (RIC)

11. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 11 HRP Support to IP Science CSA BP Reg – 33S US & CSA FE; uses HRF PFS, Continuous Blood Pressure Monitoring Device (CBPD) Vascular – 33S CSA FE; uses HRF RC ESA EKE &Thermolab – 31S US FE; performed in conjunction with VO2 Max Vessel Imaging – 31S JAXA & US FE, 33S US FE; HRF Ultrasound 2; performed in conjunction with ICV Scans Energy – 31S JAXA, 33S CSA & US FE; uses HRF PFS MARES Commissioning Part 2 – 33S US & CSA FE; Ultrasound2, and PEMS II

12. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 12 Inc. 33/34 Assumptions/Forward Work Assumptions: –ICV FD75 Exercise Echo for 30S crew is successful in the US LAB  Will support ICV FD75 Exercise Echo for both 31S crew and 33S subjects –MARES Commissioning Part 1 is completed in Increment 31/32 Forward Work –Spinal Ultrasound  ECR Submittal  Baseline of Operational Products  CHIT update for CMRS use with Spinal Protocol

13. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 13 Backup Slides: Quick Review HRP Experiment Operations

14. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 14 Crew Participation Experiment31S JAXA Hoshide 31S US Williams 32S US Ford 33S CSA Hadfield 33S US Marshburn Bisphosphonates (Control) Integrated Cardiovascular Opr Journals Nutrition Repository Pro K Reaction Self Test Spinal UltrasoundOpr Sprint Treadmill Kinematics VO2Max Pre-Post only: Functional Task Test Intervertebral Disc Damage (IVD) Manual Control

15. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 15 PRINCIPAL INVESTIGATOR Scott A. Dulchavsky, M.D., Ph.D. RESEARCH OBJECTIVES Baseline MRI of the cervical and lumbosacral vertebrae and MSK ultrasound examinations from long duration astronaut crews will be obtained preflight and compared for agreement. Comprehensive cervical and lumbar ultrasound examinations will be completed on the ISS crewmembers at early, mid, and late flight periods using Spinal Ultrasound Experiment Review software and remote expert guidance methodologies on the ISS. Follow-up MRI and ultrasound examinations will be completed within the first week post-flight for the crew members. Results will be evaluated for morphometric changes in the muscular, bony, and ligamentous vertebral unit structures at each time period. Spinal Ultrasound

16. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 16 PRINCIPAL INVESTIGATOR Dr. Alan Hargens, UCSD Medical Center, San Diego, Ca. RESEARCH OBJECTIVES The goal of this study is to use state-of-the-art imaging technologies to comprehensibly characterize and quantify space- flight induced changes in disc morphology, biochemistry, metabolism, and kinematics. Subjects will be imaged before and after prolonged spaceflight. These data will be correlated with low back pain that spontaneously arises in space so as to establish pain and disc damage mechanisms that will serve as a basis for future countermeasure development. Intervertebral Disc Damage

17. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 17 PRINCIPAL INVESTIGATOR Steven Moore, Ph.D., Mount Sinai School of Medicine, New York, NY RESEARCH OBJECTIVES Lack of gravity causes sensorimotor deficits post-landing. This experiment's comprehensive cognitive/sensorimotor test battery will determine the relative contribution of specific mechanisms (including sleepiness and fatigue) underlying decrements in post- flight operator proficiency. These results will be critical in determining whether sensorimotor countermeasures are required for piloted landings and early surface operations, and what functional areas countermeasures should target. Manual Control

18. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 18 Integrated Cardiovascular: Benjamin Levine, M.D., Michael Bungo, M.D. –Brief Research Summary: Cardiac Atrophy and Diastolic Dysfunction During and After Long Duration Spaceflight: Functional Consequences for Orthostatic Intolerance, Exercise Capability and Risk for Cardiac Arrhythmias (Integrated Cardiovascular) will quantify the extent, time course and clinical significance of cardiac atrophy (decrease in the size of the heart muscle) associated with long-duration space flight. This experiment will also identify the mechanisms of this atrophy and the functional consequences for crewmembers who will spend extended periods of time in space. –Remember: Scheduling of Ambulatory Monitoring will present a challenge. ALL USOS crew participating (only 1 Cardiopres on-board).

19. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 19 Integrated Cardiovascular (continued): –Mobile Ultrasound Scanning: Integrated Cardiovascular FD75 Exercise Echo is targeted for performance in the US LAB. The ICV experiment has a requirement of performing the exercise scan while crew is exercising on the CEVIS. –Target crew: The current target for implementation is FD75 for 30S crew (Acaba). –The plan is to use ExPRESS Rack located in US LAB as a power and video interface for remote scanning capabilities at CEVIS. –Upon completion of scan, connect Ultrasound 2 hardware to HRF Rack 1 and perform existing data transfer and downlink protocols.

20. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 20 Journals: Jack Stuster, Ph.D. –Brief Research Summary: This study converts behavioral and human factors information contained in confidential journal entries into quantitative data concerning the importance of the various behavioral issues involved in extended- duration space exploration. –Remember: First performance creates a file. Data downlinked monthly via OCA.

21. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 21 Pro K (Nutrition / Repository / Bisphosphonates (control)) Scott M. Smith, Ph.D. –Brief Research Summary: The Dietary Intake Can Predict and Protect Against Changes in Bone Metabolism during Spaceflight and Recovery investigation is NASAs first evaluation of a dietary countermeasure to lessen bone loss of astronauts. Pro K proposes that a flight diet with a decreased ratio of animal protein to potassium will lead to decreased loss of bone mineral. –Remember: If 24-hr urine collection closes out after the blood draw, the crew needs to continue diet log and mimic Pro K diet as closely as possible until the final urine insert is completed. For blood draw, S/G is preemptively privatized for Med Trainer consultation. Day 1Day 2Day 3Day 4 Controlled/Monitored (FD30) Diet Urine pH Testing (1 st Void only) Blood Draw (Fasted) 24-hr Urine Collection Day 1Day 2Day 3Day 4Day 5 Day 4Day 5 Bld s/u Day 5 pH Log Photo and Menu File Downlink Urn s/u

22. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 22 Reaction Self Test: David Dinges, Ph.D. –Brief Research Summary: The Psychomotor Vigilance Self Test on the International Space Station is a portable 5- minute reaction time task that will allow the crewmembers to monitor the daily effects of fatigue on performance while on board the International Space Station. –Remember: Performed on any SSC every 4 days, within 2 hr of waking and within 2 hr of going to sleep. Different schedule for sleep shifts and EVAs. Data downlinked monthly via OCA.

23. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 23 Sprint: Lori Ploutz-Snyder, Ph.D. –Brief Research Summary: The purpose of the Sprint study is to evaluate the efficacy of a new integrated resistance and aerobic training program designed to minimize the loss of muscle, bone, and cardiovascular function during ISS missions. –Remember: This experiment involves an exercise presciption, Sprint VO2max and Leg USND Scans.

24. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 24 Treadmill Kinematics: John DeWitt, Ph.D. –Brief Research Summary: The Treadmill Kinematics study on the International Space Station is a collect biomechanical data during treadmill exercise on the ISS to determine the appropriate speed and load combinations that may be the best to maintain bone and muscle health. –Remember: Video must be recorded in HD and MPC playback must be in HD.

25. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 25 VO2 Max: Alan Moore, Jr., Ph.D. –Brief Research Summary: Evaluation of Maximal Oxygen Uptake and Submaximal Estimates of VO2max Before, During, and After Long Duration International Space Station Missions documents changes in maximum oxygen uptake for crewmembers onboard the ISS on long-duration missions. –Remember: Strict Ku/S requirements for protocol; enablement of HRF hardware expert for direct communication with the crew is critical to minimize timeline impacts.

26. Payload Operations Integration Working Group (POIWG) July 2012 Human Research Program (HRP) Andres Ovalles 26 The Human Research Program conducts research and develops technologies that allow humans to travel safely and productively in the environment of space.