1.  All animals need to exchange substances with the environment  Diffusion › Surface area › Concentration gradient › Distance  SURFACE AREA : VOLUME › Bacteria – 6 000 000/m › Whale – 0.06/m  Maximum size limit of single cell  All organisms larger than size limit are MULTICELLULAR

2. Surface area to volume ratio gets smaller as the cell gets larger !

3. Avoidance Geometric solutions Increase surface area Decrease effective volume Increase rate of supply High concentration of nutrients Improve nutrient transport within Improve efficiency to reduce demand Division of labor within the cell Division of labor between cells

4.  Evolved many times in eukaryotes  Three theories › Symbiotic Theory  Like the endosymbiotic theory  Different species are involved › Syncytial Theory  Ciliates and slime molds  Commonly occur in multinucleated cells › Colonial Theory (Haeckel, 1874)  Same species are involved  Green algae (Chlorophyta) > 7000 species  Model: Volvocine series – Order Volvocales

6.  Unicellular flagellate  Isogamy

7. Small colony (4, 8,16, or 32 cells) Flat plane, mucilage No differentiation Isogamy Intercellular communication

8.  Colony (8, 16, or 32 cells) in 1 layer  Spherical  Isogamy  Anterior cells  larger eyespots  Coordinate flagellar movement  Colony dies when disrupted

10.  16 or 32 cells  16 cells – no specialization  32 – 4 for motility, the rest for reproduction  Heterogamy – female gametes not released  Halves are more pronounced

11.  32 to 128 cells  Heterogamy – female gametes not released, in some cases becoming truly non- motile  Division of labor › Anterior vegetative cells › Larger posterior reproductive cells

12.  Spherical colonies (500-50000 cells)  Hollow sphere – coenobium  Cell differentiation: somatic/vegetative cells and gonidia  2-50 scattered in the posterior  reproductive  Female reproductive cells  daughter colonies  Intercellular communication possible

14. Reproduction in the Volvox

16.  Unicellular  colonial life  Increase in # of cells in colonies  Change in shape of colony  Increase in interdependence among vegetative cells  Increase in division of labor: vegetative and reproductive cells  Isogamy  anisogamy  oogamy  Fewer female gametes are produced

17.  Increase in size of the organism  Permits cell specialization  Increase in surface area to volume ratio

18.  Interdependence  Complexity

19.  http://protist.i.hosei.ac.jp/pdb/images/Chlo rophyta/Gonium/pectorale/sp_2b.jpg http://protist.i.hosei.ac.jp/pdb/images/Chlo rophyta/Gonium/pectorale/sp_2b.jpg  http://www.rbgsyd.nsw.gov.au/__data/asse ts/image/48212/Gonium2.gif http://www.rbgsyd.nsw.gov.au/__data/asse ts/image/48212/Gonium2.gif  http://www.ac- rennes.fr/pedagogie/svt/photo/microalg/p andorin.jpg http://www.ac- rennes.fr/pedagogie/svt/photo/microalg/p andorin.jpg  http://protist.i.hosei.ac.jp/PDB/images/Chlo rophyta/Eudorina/elegans/sp_5.jpg http://protist.i.hosei.ac.jp/PDB/images/Chlo rophyta/Eudorina/elegans/sp_5.jpg  http://www.fytoplankton.cz/FytoAtlas/thm/ 0078.jpg http://www.fytoplankton.cz/FytoAtlas/thm/ 0078.jpg