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Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing  Currently, laboratories use automated DNA sequencing to determine the unknown.

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Presentation on theme: "Automated DNA Sequencing AP Biology Fall 2010. Automated DNA Sequencing  Currently, laboratories use automated DNA sequencing to determine the unknown."— Presentation transcript:

1 Automated DNA Sequencing AP Biology Fall 2010

2 Automated DNA Sequencing  Currently, laboratories use automated DNA sequencing to determine the unknown sequence of bases in a DNA sample

3 Automated DNA Sequencing  The machine builds DNA molecules but uses eight kinds of bases Four normal Four that are modified to fluoresce in laser light

4 Automated DNA Sequencing  When a modified base is incorporated, DNA synthesis is halted This produces tagged fragments of different lengths

5

6 Gel Electrophoresis  The automated DNA sequencer separates the sets of fragments by gel electrophoresis

7 Gel Electrophoresis  The “tag” base at the end of each fragment in the set is identified by the laser beam  The computer program in the machine assembles the information from all the nucleotides in the sample to reveal the entire DNA sequence  http://www.youtube.com/watch?v=oYpllb I0qF8 http://www.youtube.com/watch?v=oYpllb I0qF8

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