1. Soil Microbiology Group Soil Microbial communities from Puerto Rico rain forest (that decompose switchgrass, sample from Feedstock-adapted consortia SG and SG+Fe) Alinne Castro – University of Brasilia Jessica C. Bergmann – JBEI Vara Kensakurti Samba Si – Agriculture & Agri-Food Canada

2. Overview Introduction Where this soil came from? What is the difference between them? Comparison of the two samples What are the populations in the two metagenomes? Are the two populations functionally different from each other? Is there any Glycosyl Hydrolases (GHs) in these samples?

3. Where this soils came from? Environment: Soil from Puerto Rico Rain Forest (Luquillo) Soil collected April 2010 Sequenced in 454 GS-FLX-Titanium

4. Samples – Gene Count

5. What is the difference between the two soils? SG – Switchgrass (carbon source) SG + Fe – Switchgrass enriched with iron Iron addition – increase diversity of feedstock – cultures limited for electron donor

6. Overview Introduction Where this soil came from? What is the difference between them? Comparison of the two samples What are the populations in the two metagenomes? Are the two populations functionally different from each other? Is there any Glycosyl Hydrolases (GHs) in these samples?

7. What are the populations in the two metagenomes? Compare Genomes (Metagenome X Genome) Percent Identity – 90% - Phylum SG SG + Fe

8. SG Order Compare Genomes – Phylogenic Distribution - Radial Tree

9. Functional Difference 54 significant Pfam function Analysis Cart – Profile and Alignment – View Function vs. Genome

10. Is there any Glycosyl Hydrolases (GHs) in these samples?

11. IMG – Find Function – Save to Function Cart – Profile and Alignment Tool

12. Thank you !