1. In the name of God Summer School Influenza Unit, Pasteur Institute of Iran 1

2. Practical approach in virology; Virus quantification method A. Abdoli Sh. Gholami P. Biglari H. Sohani H. Rahmatollahi M. Abdoli Influenza Unit, Pasteur Institute of Iran Summer School

3. Outline of the lecture 2 fold dilution 10 fold dilution Multiplicity of infection(moi) Hemagglination assay (HA) hemagglutination inhibition assay (HI) Cell culture infective dose %50 (CCID50) Plaque Assay fluorescent-focus assay Influenza Unit, Pasteur Institute of Iran summer 2011 3

4. Hemagglutination assay Influenza Unit, Pasteur Institute of Iran summer 2011 4

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6. TCID50 6

7. Plaque Assay 7

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9. cytopathic effect of virus in pfu 9

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11. Principal of fluorescent assay 11

12. fluorescent-focus assay 12