1. 1 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Human Research Facility Pulmonary Function System (HRF PFS) Hardware Overview & Task Training Instructors: Martin Bost281.483.9555martin.bost@nasa.gov Erik Hougland281.244.1553erik.a.hougland@nasa.gov

2. 2 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Identify the major components and functions of the Pulmonary Function System (PFS): Pulmonary Function Module/Photoacoustic Analyzer Module (PFM/PAM) Gas Delivery System (GDS) Rebreathing Hardware and Mixing Bag System (MBS) Provide hands-on exposure to the basic hardware configurations. Provide hands-on exposure to the PFS software. Lesson Objectives

3. 3 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center The PFS is a NASA/ESA collaborative development in the field of respiratory physiology instrumentation. Two primary components: HRF PFM/PAM & HRF GDS Capable of wide range gas analysis using rebreathing protocols and gas mixing. Current Activities Overview Maintenance: –Hardware Calibration –Syringe Maintenance –Relief Valve Checkout –Tank gauge photos Experiments: –BP Regulation (rebreathing) –Energy (MBS)

4. 4 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Training vs. Flight Hardware The flight hardware has a camera shoe attachment for mounting to the seat track using a flexible bracket. –Respiratory Valve Unit –Mixing Bag System Main Body –The pole used in training is for 1-g mounting only. All on-orbit cables and connectors will have caps. All cables are properly labeled on orbit

5. 5 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Pulmonary Function Module (PFM) –Gas Management System –Data Management Unit –Electronics –Requires external PC to operate Photoacoustic Analyzer Module (PAM) –Two miniaturized Photoacoustic Gas Analyzer(s) (PGA) –One laser-based Oxigraf ® oxygen analyzer –Analyzers require 15 – 45 minutes to warm up HRF PFM/PAM

6. 6 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center HRF PFM/PAM Interfaces POWER switch ON/OFF SELECTOR Rear = Rack Power Front = Front Power ANALOG I/O J504 Used for Inlet Switching Box GO SWITCH J503 Connection for Go Switch CALIBRATION PORT Calibration gas output port GAS INLET Gas sampling port ACMS J506 Connection for ACMS BARGRAPH DISPLAY J510 Connection for Bargraph Display FLOWMETER J507 Connection for RFM Box RVU J511 Connection for RVU Cable BAG FILLING Connection for Bag Filling Assembly GAS INPUTS Gas input ports for connections to HRF GDS Common Interfaces Rebreathing Specific Mixing Bag Specific

7. 7 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center External Hardware Connections FRONT POWER J101 Used to power from external power source auxiliary data connections CBPD J502 Connection for Continuous Blood Pressure Device (CBPD) RIP J505 Connection for Respiratory Inductive Plethysmograph (RIP) EXTERNAL BAG FILLING Connection for non-PFS bag filling GASMAP DATA J508 Connection for Gas Analyzer System for Metabolic Analysis Physiology (GASMAP) PC DATA J512 Connection for external PC GAS OUTLET Gas outlet port NOTE: The Continuous Blood Pressure Device (CBPD) is currently the only external hardware system that is being used with the HRF PFM/PAM

8. 8 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center HRF GDS Supplies calibration and respiratory gases to the HRF PFM/PAM Holds five 1.3 L cylinders –2400 psig max (leak before burst) working pressure –Relief valves are two fault tolerant –No safety hazard if gasses leaked into the cabin –Cylinders are replaceable Tank pressures visible on front of unit (use gauge guide to read)

9. 9 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center GDS Tank Contents Tank 1 15% Oxygen (O 2 ) 85% Nitrogen (N 2 ) Tank 2 10% Carbon Dioxide (CO 2 ) 1% Argon (Ar) 89% Oxygen (O 2 ) Tank 3 Spare tank Contents may vary Tanks 4 & 5 71.2% Nitrogen (N 2 ) 0.3% Carbon Monoxide (CO) 0.5% Acetylene (C 2 H 2 ) 1.0% Sulfur Hexafloride (SF 6 ) 1.0% Freon (R-22) 1.0% Methane (CH 4 ) 25% Oxygen (O 2 )

10. 10 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Unique Characteristics –4L Rebreathing Bag is filled with a gas mixture from the GDS –Subjects breathe in and out of the rebreathing bag –Gas composition in the bag changes over time –O 2 decreased while CO 2 increases –Blood-soluble gases will decrease while insoluble gases will not –Used to assess various parameters of pulmonary, as well as cardiac, function (e.g., lung volume, cardiac output, etc.). Basic Session Flow –Hardware assembly –PFM/PAM power on and warm-up –Calibration and experiment protocol –Hardware disassembly and cleaning Rebreathing Hardware Overview

11. 11 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Other PFS Stowage PFS Accessories Kit 2 Bargraph Display Mount COL1A4: HRF Rack 2 HRF PFS Accessories Kit HRF Gas Delivery System HRF PFM/PAM PFS Stowage Drawer Rebreathing Hardware Experiment-Provided Consumables Integrated Sample Catheter Mouthpiece Saliva Trap 4L Rebreathing Bag Biocide Wipes Dry Wipes

12. 12 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center HRF PFM/PAM Bag Filling Assembly HRF PFM/PAM RVU Cable HRF PFM/PAM Respiratory Valve Unit (RVU) PFS Stowage Drawer - Rebreathing HRF PFM/PAM Bargraph Display HRF PFM/PAM ACMS HRF PFM/PAM Go Switch

13. 13 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Bacterial Filters (if replacement required) HRF PFM/PAM GDS Hose (x3) Nose Clips PFS Accessories Kit - MBS

14. 14 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center 4L Rebreathing Bag Mouthpiece Saliva Trap Integrated Sample Catheter Dry Wipes Biocide Wipes Basic Consumables - Rebreathing

15. 15 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Rebreathing Basic Configuration Mouthpiece Saliva Trap 4L Rebreathing Bag Bag Filling Assembly Bargraph Display Mount GDS Hose connections to GDS

16. 16 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Unique Characteristics –Subjects breathe in cabin air and exhale into a large (15 L) bag –The composition of inhaled gas (fairly constant) is measured and compared to that of the exhaled gas (which is mixed over time, not breath-by-breath) –Used for measuring aerobic capacity and oxygen uptake Basic Session Flow –Hardware assembly –PFM/PAM power on and warm-up –Calibration and experiment protocol –Hardware disassembly and cleaning –Expiration Hose set aside to dry and stowed at end of crew day Mixing Bag System (MBS) Overview

17. 17 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Other PFS Stowage PFS Accessories Kit 2 COL1A4: HRF Rack 2 HRF PFS Accessories Kit HRF PFM/PAM Calibration Syringe Tube PuFF Volume Calibration Syringe HRF Gas Delivery System HRF PFM/PAM PFS Stowage Drawer MBS Hardware Experiment-Provided Consumables Integrated Sample Catheter Mouthpiece Saliva Trap DPFM Screens Biocide Wipes Dry Wipes

18. 18 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center HRF PFM/PAM RFM Box HRF PFM/PAM RVU Cable HRF PFM/PAM DPFM 1 PFS Stowage Drawer - MBS HRF PFM/PAM Bargraph Display HRF PFM/PAM ACMS HRF PFM/PAM Go Switch

19. 19 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Bacterial Filters (if replacement required) HRF PFM/PAM GDS Hose (x3) Nose Clips PFS Accessories Kit - MBS

20. 20 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center PFS Accessories Kit 2 - MBS 15L Bag MBS Breathing Valve Connection Inspiration Hose, Expiration Hose Inner & Outer Tubes MBS Main Body Mouthpiece Interface Inlet Switching Box Bargraph Display Mount

21. 21 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Integrated Sample Catheter Mouthpiece Saliva Trap DPFM Screens Biocide Wipes Dry Wipes Basic Consumables - MBS

22. 22 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center MBS Basic Configuration Inlet Switching Box

23. 23 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center MBS Catheter Configuration Catheter measuring exhaled gases (mixed, not breath- by-breath) Catheter measuring inhaled gases

24. 24 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center Hardware Cleanup Clean accessible surfaces with Biocide Wipe Dry accessible surfaces with Dry Wipe 15L Bag Reverse the bag to access the inner surface Expiration Hose Wipe and dry accessible opening then place in a high air flow area of the cabin to dry Previously used solution: Place hose opening in front of a vent

25. 25 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center PFS Software Protocols for breathing tests and calibrations are prompted by the PFS software. –PODF or SODF procedures will include steps to set-up hardware. –P/SODF procedures will instruct the crewmember to follow the on- screen instructions for calibrations and experiment protocols. –On-screen instructions may point back to P/SODF for additional information or hardware setup. On-screen instructions coordinate subject and hardware actions. –Timed breathing –Variations in controlled breathing

26. 26 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center PFS Software - Basic Interface On-screen Instructions Active Step (“  ” indicator) Go Switch can be used to “Press” outlined button Software Buttons NOTE: Any required deviations from on-screen instructions will be listed in a Note box before the affected step in P/SODF procedures. Completed Step (“ X ” indicator)

27. 27 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center PFS Software - Step Displays Data Display On-Screen Step Instructions

28. 28 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center PFS Software - Calibration NOTE: The PAM Calibration has a unique display for instructions outside of the typical on-screen instruction block. On-screen Instructions Calibration Instructions Software Buttons

29. 29 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center ACMSAmbient Conditions Monitoring System CBPDContinuous Blood Pressure Device DPFMDifferential Pressure Flowmeter GDSGas Delivery System HRFHuman Research Facility LLiter MBS Mixing Bag System PAMPhotoacoustic Analyzer Module PCPortable Computer PFMPulmonary Function Module PFSPulmonary Function System PGAPhotoacoustic Gas Analyzer PODFPayload Operations Data File PPFSPortable Pulmonary Function System psigpounds per square inch gauge List of Acronyms

30. 30 Hardware Overview & Task Training 2013Martin Bost / Erik Hougland Pulmonary Function System Lyndon B. Johnson Space Center List of Acronyms, cont. RFMRespiratory Flowmeter RIPRespiratory Inductive Plethysmograph RVU Respiratory Valve Unit SODFSystem Operations Data File