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pGLO Bacterial Genetics
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E. Coli structure
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Plasmid Additional DNA found in some bacteria Small loop Carries one or more genes Can be transferred from one bacteria to another.
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pGLO plasmid (original form) ori- point of origin for DNA replication araC- gene for repressor protein in arabinose operon araB, A and D- gene for arabinose metabolism ara B, ara A & ara D
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pGLO plasmid (engineered form) Gene for arabinose metabolism (ara BAD) has been replaced with GFP. Amipicillin resistance has been added (bla)
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